肠道病毒70型病毒体及细胞内蛋白。
Enterovirus type 70 virion and intracellular proteins.
作者信息
Esposito J J, Obljeski J F
出版信息
J Virol. 1976 Jun;18(3):1160-2. doi: 10.1128/JVI.18.3.1160-1162.1976.
Abstract
The proteins of purified enterovirus type 70 grown in rhabdomyosarcoma cells and the intracellular proteins at 4 to 5 h after infection have been examined by polyacrylamide gel electrophoresis. Virions contained four proteins: P-1 (35,000, daltons) P-2 (28,000, daltons) P-3 (27,000, daltons) and P-4 (9,000 daltons). Further, addition of ZnCl2 to infected cultures inhibited virus plaque development and interfered with post-translational cleavage.
摘要
利用聚丙烯酰胺凝胶电泳对在横纹肌肉瘤细胞中培养的纯化70型肠道病毒的蛋白质以及感染后4至5小时的细胞内蛋白质进行了检测。病毒粒子包含四种蛋白质：P-1（35,000道尔顿）、P-2（28,000道尔顿）、P-3（27,000道尔顿）和P-4（9,000道尔顿）。此外，向受感染培养物中添加氯化锌会抑制病毒空斑形成，并干扰翻译后切割。
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