急性出血性结膜炎病毒的纯化及生物物理特性
Purification and biophysical properties of acute haemorrhagic conjunctivitis virus.
作者信息
Yamazaki S, Natori K, Kono R
出版信息
J Virol. 1974 Dec;14(6):1357-60. doi: 10.1128/JVI.14.6.1357-1360.1974.
Abstract
The buoyant density of acute haemorrhagic conjunctivitis virions labeled with either [(3)H]uridine or [(3)H]leucine was 1.34 g/ml in CsCl and 1.25 g/ml in sucrose. RNA extracted from the virions gave a sedimentation coefficient of approximately 34S in sucrose, and was found to be sensitive to RNase. Molecular weight of RNA was calculated to be 2.5 x 10(6) using poliovirus RNA for reference.
摘要
用[³H]尿苷或[³H]亮氨酸标记的急性出血性结膜炎病毒粒子在氯化铯中的浮力密度为1.34 g/ml，在蔗糖中的浮力密度为1.25 g/ml。从病毒粒子中提取的RNA在蔗糖中的沉降系数约为34S，并且发现其对核糖核酸酶敏感。以脊髓灰质炎病毒RNA为参照，计算出RNA的分子量为2.5×10⁶。
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