Alvarez-Arellano Lourdes, Camorlinga-Ponce Margarita, Maldonado-Bernal Carmen, Torres Javier
Infectious Diseases Research Unit, Hospital de Pediatría, Centro Médico Nacional SXXI, IMSS, Mexico City, México.
FEMS Immunol Med Microbiol. 2007 Dec;51(3):473-9. doi: 10.1111/j.1574-695X.2007.00327.x. Epub 2007 Sep 24.
The innate immune response to Helicobacter pylori infection is beginning to be understood and recent works support a role for Toll-like receptors (TLRs). Our aim was to study the response of human neutrophils to H. pylori and to elucidate the role of TLR2 and TLR4. Neutrophils from healthy H. pylori-negative volunteers were cocultured with H. pylori 26695 strain. The release of IL-8, IL-1beta, tumor necrosis factor (TNF)-alpha and IL-10 was measured. The role of TLR2 and TLR4 was investigated with blocking assays using monoclonal antibodies against TLRs. Neutrophils produced a significant increase of IL-8, IL-1beta and TNF-alpha after 3, 6 and 24 h of H. pylori challenge, respectively, whereas IL-10 increased after 24 h. Helicobacter pylori challenge increased TLR2 and TLR4 expression; and antibodies against TLR2 and TLR4 diminished significantly the H. pylori-induced production of IL-8 and IL-10. In human neutrophils, H. pylori induces an early inflammatory response, partially mediated via TLR2 and TLR4 activation.
对幽门螺杆菌感染的先天性免疫反应正逐渐被人们所了解，近期的研究支持Toll样受体（TLR）发挥作用。我们的目的是研究人类中性粒细胞对幽门螺杆菌的反应，并阐明TLR2和TLR4的作用。将来自健康幽门螺杆菌阴性志愿者的中性粒细胞与幽门螺杆菌26695菌株共培养。检测白细胞介素-8（IL-8）、白细胞介素-1β（IL-1β）、肿瘤坏死因子（TNF）-α和白细胞介素-10（IL-10）的释放情况。使用针对TLR的单克隆抗体通过阻断试验研究TLR2和TLR4的作用。幽门螺杆菌攻击3、6和24小时后，中性粒细胞分别显著增加了IL-8、IL-1β和TNF-α的产生，而IL-10在24小时后增加。幽门螺杆菌攻击增加了TLR2和TLR4的表达；针对TLR2和TLR4的抗体显著减少了幽门螺杆菌诱导的IL-8和IL-10的产生。在人类中性粒细胞中，幽门螺杆菌诱导早期炎症反应，部分通过TLR2和TLR4激活介导。
