Añón M C, Grau O, Segovia Z M, Franzefernández M T
J Virol. 1976 Jun;18(3):833-8. doi: 10.1128/JVI.18.3.833-838.1976.
Junin virus grown in BHK-21 cells was labeled with [3H]uridine and highly purified by differential and isopycnic centrifugation. The RNAs extracted with phenol and analyzed by polyacrylamide gel electrophoresis were shown to be composed of four large species (33, 28, and 18S) and three small ones (4, 5, and 5.5S). This pattern was similar to ones already reported for other arenaviruses. However, there was a striking difference when the virus labeling was performed in the presence of actinomycin D. Labeling of viral rRNA was as much as 60% of the levels obtained in the absence of the drug under conditions in which cellular rRNA's were inhibited by 95% or more.
在BHK - 21细胞中培养的胡宁病毒用[³H]尿苷标记，并通过差速离心和等密度离心进行高度纯化。用苯酚提取并通过聚丙烯酰胺凝胶电泳分析的RNA显示由四种大分子（33、28和18S）和三种小分子（4、5和5.5S）组成。这种模式与已报道的其他沙粒病毒的模式相似。然而，当在放线菌素D存在的情况下进行病毒标记时，存在显著差异。在细胞rRNA被抑制95%或更多的条件下，病毒rRNA的标记量高达在无该药物情况下获得水平的60%。
