Borges Maria Beatriz J, Kato Sayuri E M, Damaso Clarissa R A, Moussatché Nissin, da Silva Freire Marcos, Lambert Passos Sonia Regina, do Nascimento Jussara Pereira
Vice-diretoria de Desenvolvimento Tecnológico, Bio-Manguinhos/FIOCRUZ, Avenida Brasil 4365, Manguinhos, 21040-900 Rio de Janeiro, RJ, Brazil.
Biologicals. 2008 Mar;36(2):105-10. doi: 10.1016/j.biologicals.2007.07.001. Epub 2007 Sep 24.
The detection of neutralizing antibodies against vaccinia virus is a valuable tool for the investigation of previous smallpox vaccination. Compulsory smallpox vaccination ended in Brazil during the early 1970s, although the vaccine was available until the late 1970s. The threat of smallpox as a biological weapon has called the attention of public health authorities to the need for an evaluation of the immune status of the population. Based on our previous experience with a micro plaque reduction neutralization test (PRNT) for the evaluation of yellow fever immunity, a similar test was developed for the detection and quantification of vaccinia neutralizing antibodies. A cross-sectional study to test the repeatability and validity of plaque reduction neutralization test (PRNT) for vaccinia antibodies was performed in 182 subjects divided into two categories: subjects above 31 years old and the other > or = 35 years old. Cases were subjects considered to have been vaccinated with vaccinia virus if they declared vaccination history or evidenced vaccination marks. The assay is carried out in 96-well plates, provides results within 30 h, is easily performed, has good sensitivity (92.7%) and specificity (90.8), excellent repeatability (ICC 0.89 (0.88; 0.92)) and is thus suitable for use in mass screening of a population's antibody levels.
检测抗痘苗病毒中和抗体是调查既往天花疫苗接种情况的一项重要工具。20世纪70年代初，巴西停止了强制性天花疫苗接种，不过该疫苗一直供应到70年代末。天花作为生物武器的威胁已引起公共卫生当局对评估人群免疫状况必要性的关注。基于我们之前使用微量空斑减少中和试验（PRNT）评估黄热病免疫力的经验，开发了一种类似的试验用于检测和定量痘苗中和抗体。对182名受试者进行了一项横断面研究，以测试痘苗抗体空斑减少中和试验（PRNT）的重复性和有效性，这些受试者分为两类：31岁以上的受试者和另一组年龄≥35岁的受试者。如果受试者申报有疫苗接种史或有疫苗接种痕迹，则被视为接种过痘苗病毒的病例。该检测在96孔板中进行，30小时内出结果，操作简便，具有良好的敏感性（92.7%）和特异性（90.8%），重复性极佳（组内相关系数ICC为0.89（0.88；0.92）），因此适用于大规模筛查人群的抗体水平。
