Van Deun Kim, Haesebrouck Freddy, Heyndrickx Marc, Favoreel Herman, Dewulf Jeroen, Ceelen Liesbeth, Dumez Linn, Messens Winy, Leleu Saskia, Van Immerseel Filip, Ducatelle Richard, Pasmans Frank
Department of Pathology, Bacteriology and Avian Diseases, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Unit, Brusselsesteenweg 370, 9090 Melle, Belgium.
J Med Microbiol. 2007 Oct;56(Pt 10):1284-1289. doi: 10.1099/jmm.0.47342-0.
Campylobacter jejuni is one of the leading causes of food-borne gastroenteritis. Because of the high prevalence of C. jejuni in poultry, poultry meat is considered a major source of C. jejuni infections for humans. However, it is not known whether all poultry-associated C. jejuni strains are capable of causing disease in humans. Four different virulence properties of C. jejuni strains were compared between 20 poultry isolates and 24 human isolates. Strains were chosen based on their PFGE pattern to represent a heterogeneous population. The isolates were compared for their ability to invade and induce interleukin-8 (IL-8) production in T84 cells, their production of functional cytolethal distending toxin (CDT) using HEp-2 cells, and their sodium deoxycholate resistance. All four virulence factors were present among strains of human and poultry origin, with strong differences observed among strains. For invasion and IL-8 induction, no difference was observed between the two populations. However, on average, human isolates arrested more HEp-2 cells in their cell cycle than did the poultry isolates (P=0.041), suggesting higher CDT production by the former. The ability to survive 16 000 mug sodium deoxycholate ml(-1) was significantly more pronounced (P=0.006) among human isolates than poultry isolates, although all strains possessed the cmeABC operon. These data suggest that all four virulence properties are widespread among C. jejuni isolates, but that a higher degree of bile-salt resistance and more pronounced CDT production are associated with strains causing enteritis in humans.
空肠弯曲菌是食源性肠胃炎的主要病因之一。由于空肠弯曲菌在家禽中普遍存在，禽肉被认为是人类感染空肠弯曲菌的主要来源。然而，尚不清楚所有与家禽相关的空肠弯曲菌菌株是否都能导致人类发病。比较了20株家禽分离株和24株人类分离株的空肠弯曲菌菌株的四种不同毒力特性。根据脉冲场凝胶电泳（PFGE）图谱选择菌株以代表异质群体。比较了这些分离株在T84细胞中侵袭和诱导白细胞介素-8（IL-8）产生的能力、使用人喉表皮样癌细胞（HEp-2细胞）产生功能性细胞致死性膨胀毒素（CDT）的能力以及它们对脱氧胆酸钠的抗性。在人和家禽来源的菌株中均存在所有四种毒力因子，且菌株间观察到强烈差异。对于侵袭和IL-8诱导，在这两个群体之间未观察到差异。然而，平均而言，人类分离株比家禽分离株使更多的HEp-2细胞停滞在细胞周期中（P = 0.041），这表明前者产生的CDT更高。尽管所有菌株都拥有cmeABC操纵子，但人类分离株在16000μg脱氧胆酸钠ml⁻¹中存活的能力比家禽分离株更明显（P = 0.006）。这些数据表明，所有四种毒力特性在空肠弯曲菌分离株中广泛存在，但更高程度的胆盐抗性和更明显的CDT产生与导致人类肠炎的菌株相关。
