Chansiripornchai Niwat, Sasipreeyajan Jiroj
Department of Veterinary Medicine, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
J Vet Med Sci. 2009 Jun;71(6):839-44. doi: 10.1292/jvms.71.839.
Campylobacter jejuni is a major cause of food borne pathogens in humans and a major reservoir for this pathogen is poultry. The C. jejuni in broilers was investigated from in the caeca of broilers. Twenty broiler/flock samples from 7 flocks were assessed. The average prevalence of C. jejuni was 65% in the broiler flocks. The adhesion and invasion ability of 48 strains of C. jejuni on INT 407 were studied. The adhesion and invasion ability of 48 Campylobacter isolates from caecal contents were analyzed with Human embryonic intestine (INT-407) cells being used as a gentamicin resistance assay. The caecal isolates exhibited a wide range of adherence and invasion ability. There was a significant correlation (p<0.01) between the adherence and the invasion ability of the Campylobacter isolates. Each of the virulence-associated genes: dnaJ, cadF, pldA and ciaB was detected by polymerase chain reaction from 100, 76, 31 and 41% of the Campylobacter strains, respectively. All of four virulence-associated genes were detected in 11 isolates. However, there was unclear association between the invasion ability and the presence of virulence-associated genes in this experiment, suggesting that more genes may be involved in the invasion process.
空肠弯曲菌是人类食源性病原体的主要病因，这种病原体的一个主要宿主是家禽。对肉鸡盲肠中的空肠弯曲菌进行了调查。评估了来自7个鸡群的20个肉鸡/鸡群样本。空肠弯曲菌在肉鸡群中的平均流行率为65%。研究了48株空肠弯曲菌对INT 407的黏附与侵袭能力。以人胚肠（INT-407）细胞作为庆大霉素抗性检测对象，分析了48株来自盲肠内容物的弯曲菌分离株的黏附与侵袭能力。盲肠分离株表现出广泛的黏附与侵袭能力。弯曲菌分离株的黏附与侵袭能力之间存在显著相关性（p<0.01）。分别通过聚合酶链反应从100%、76%、31%和41%的弯曲菌菌株中检测到每个与毒力相关的基因：dnaJ、cadF、pldA和ciaB。在11株分离株中检测到所有四个与毒力相关的基因。然而，在本实验中，侵袭能力与毒力相关基因的存在之间的关联尚不清楚，这表明可能有更多基因参与侵袭过程。
