Wang Fei, Stewart James McD, Zhang Jinfa
Department of Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM 88003, USA.
Genome. 2007 Sep;50(9):818-24. doi: 10.1139/g07-061.
Cytoplasmic male sterility (CMS) is a maternally inherited trait in which plants do not produce viable pollen. Fertility in plants with CMS can be recovered by nuclear restorer genes. Most restorer genes cloned so far are members of the pentatricopeptide repeat (PPR) protein family. The objective of our study was to use the CMS-D8 and restoration (Rf2) system of cotton (Gossypium hirsutum L.) to develop more DNA markers for the Rf2 gene. In a backcross population with 112 plants, segregation of male fertility was 1 fertile : 1 sterile. Three new RAPD markers were identified for Rf2, one of which was converted to a CAPS marker. In addition, 2 AFLP markers and 1 SSR marker were identified to be linked to the fertility restorer gene (Rf2). PPR motif primers were designed based on the conserved PPR motifs and used in combination with AFLP primers to test the mapping population, and 1 PPR-AFLP marker was identified. A linkage map with 9 flanking markers including 1 from a previous study was constructed.
细胞质雄性不育（CMS）是一种母系遗传性状，具有该性状的植物不能产生可育花粉。具有CMS的植物的育性可通过核恢复基因得以恢复。目前克隆的大多数恢复基因都是五肽重复（PPR）蛋白家族的成员。我们研究的目的是利用棉花（陆地棉）的CMS-D8和恢复系（Rf2）系统，为Rf2基因开发更多的DNA标记。在一个包含112株植株的回交群体中，雄性育性的分离比例为1可育∶1不育。为Rf2鉴定出3个新的RAPD标记，其中一个被转化为CAPS标记。此外，还鉴定出2个AFLP标记和1个SSR标记与育性恢复基因（Rf2）连锁。基于保守的PPR基序设计了PPR基序引物，并将其与AFLP引物结合用于检测作图群体，鉴定出1个PPR-AFLP标记。构建了一个包含9个侧翼标记的连锁图谱，其中1个标记来自之前的研究。
