Fujii Sota, Toriyama Kinya
Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan.
Theor Appl Genet. 2005 Aug;111(4):696-701. doi: 10.1007/s00122-005-2054-0. Epub 2005 Jun 10.
Cytoplasmic male sterility (CMS) of rice (Oryza sativa L.) was first reported using the cytoplasm of a Chinese wild rice, Oryza rufipogon Griff. strain W1. However, it was not possible to characterize this ms-CW-type CMS in more detail until a restorer line had been developed due to the lack of restorer genes among cultivars thus far tested. The breeding of a restorer line (W1-R) was eventually achieved by transferring the restorer gene(s) of W1 to a cultivar. We report here the characterization of the ms-CW pollen grains and mapping of the restorer gene for ms-CW-type CMS. Pollen grains of the male-sterile plants appeared to be normal and viable based on the fluorochromatic reaction test, but they did not germinate on normal stigmas. The 1:1 segregation of fertile and sterile plants in a BC(1)F(1) population from a cross between W1-R and a maintainer line demonstrated that fertility restoration is controlled by a single gene. The fertile seed set of all the F(2) plants examined indicated that the fertility restoration functions gametophytically. We designated the fertility restorer gene Rfcw. Using cleaved amplified polymorphic sequence (CAPS) and simple sequence repeat (SSR) markers, we localized Rfcw to chromosome 4 with a genetic distance of 0.6 cM from the nearest SSR marker.
水稻（Oryza sativa L.）的细胞质雄性不育（CMS）最早是利用中国野生稻普通野生稻（Oryza rufipogon Griff.）品系W1的细胞质报道的。然而，由于迄今为止所测试的品种中缺乏恢复基因，在培育出恢复系之前，无法更详细地鉴定这种ms-CW型CMS。最终通过将W1的恢复基因转移到一个品种中，培育出了一个恢复系（W1-R）。我们在此报告ms-CW花粉粒的特征以及ms-CW型CMS恢复基因的定位。基于荧光染色反应测试，雄性不育植株的花粉粒看起来正常且有活力，但它们在正常柱头上不萌发。W1-R与一个保持系杂交的BC(1)F(1)群体中可育和不育植株的1:1分离表明育性恢复由单个基因控制。所有检测的F(2)植株的可育结实表明育性恢复是配子体遗传的。我们将育性恢复基因命名为Rfcw。利用酶切扩增多态性序列（CAPS）和简单序列重复（SSR）标记，我们将Rfcw定位到第4染色体上，与最近的SSR标记的遗传距离为0.6 cM。
