Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, 450000, Henan, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
Mol Biol Rep. 2020 Feb;47(2):1275-1282. doi: 10.1007/s11033-019-05240-5. Epub 2020 Jan 1.
The cytoplasmic male sterility (CMS) system is a useful tool for commercial hybrid cotton seed production. Two main CMS systems, CMS-D8 and CMS-D2, have been recognized with Rf and Rf as the restorer genes, respectively. The development of molecular markers tightly linked with restorer genes can facilitate the breeding of restorer lines. In this study, the InDel-1892 marker was developed to distinguish Rf and Rf simultaneously. Sequence alignment implied that CMS-D8-Rf has a 32 bp insertion and that CMS-D2-Rf has a 186 bp insertion at the InDel-1892 locus. The codominant marker was co-segregated with Rf and Rf. Hence, this marker can be used for tracing Rf and Rf simultaneously and identifying the allele status at the restorer gene locus. The results of this study will facilitate efficient marker-assisted selection for restorer lines and hybrids of CMS systems.
细胞质雄性不育（CMS）系统是商业杂交棉种生产的有用工具。已经认识到两个主要的 CMS 系统，CMS-D8 和 CMS-D2，它们分别具有 Rf 和 Rf 作为恢复基因。与恢复基因紧密连锁的分子标记的开发可以促进恢复系的培育。在这项研究中，开发了 InDel-1892 标记来同时区分 Rf 和 Rf。序列比对表明，CMS-D8-Rf 在 InDel-1892 位点有 32 个碱基对的插入，而 CMS-D2-Rf 有 186 个碱基对的插入。共显性标记与 Rf 和 Rf 共分离。因此，该标记可用于同时追踪 Rf 和 Rf，并鉴定恢复基因座的等位基因状态。本研究的结果将有助于 CMS 系统的恢复系和杂种的高效标记辅助选择。
