Schneider R P, Lynch M J, Ericson J F, Fouda H G
Drug Metabolism Department, Central Research Division, Pfizer Inc., Groton, Connecticut 06340.
Anal Chem. 1991 Sep 1;63(17):1789-94. doi: 10.1021/ac00017a024.
Pneumatically assisted electrospray mass spectrometry of polyether ionophores yields several molecular ions. A single metal adduct molecular ion can be obtained by the addition of a neutral salt to the HPLC mobile phase. This approach may be useful in structural studies of unknown ionophores and in the development of specific methods for their analysis in complex matrices. Collision-induced dissociation of the molecular ions provides additional structural information and enhanced specificity for trace analysis. HPLC mobile-phase composition and flow rates have been optimized for on-line analysis. Best response and lowest background noise were obtained at the flow rate of 40 microL/min of a mobile phase containing a 20/80 mixture of water and acetonitrile. The development of a specific confirmatory assay for the new ionophore semduramicin in chicken liver demonstrates the usefulness of on-line HPLC pneumatically assisted electrospray mass spectrometry.
聚醚离子载体的气动辅助电喷雾质谱会产生多个分子离子。通过向高效液相色谱（HPLC）流动相中添加中性盐，可以获得单一的金属加合分子离子。这种方法可能有助于未知离子载体的结构研究以及开发在复杂基质中对其进行分析的特定方法。分子离子的碰撞诱导解离可提供额外的结构信息，并增强痕量分析的特异性。已对HPLC流动相组成和流速进行了优化以用于在线分析。在含有水和乙腈20/80混合物的流动相流速为40微升/分钟时，获得了最佳响应和最低背景噪声。针对鸡肝中新离子载体塞杜霉素开发的特定确证分析方法证明了在线HPLC气动辅助电喷雾质谱的实用性。
