采用香草醛柱后衍生液相色谱法测定家禽肝脏中的赛杜霉素钠。
Determination of semduramicin sodium in poultry liver by liquid chromatography with vanillin postcolumn derivatization.
作者信息
Ericson J F, Calcagni A, Lynch M J
机构信息
Pfizer Inc., Drug Metabolism Department, Groton, CT 06340.
出版信息
J AOAC Int. 1994 May-Jun;77(3):577-82.
A liquid chromatographic (LC) method is described for the quantitative determination of semduramicin sodium in broiler liver when administered under projected use conditions. For this procedure, semduramicin sodium is extracted from liver with methanolic ammonium hydroxide, separated and concentrated by solid-phase extraction steps, and determined by LC with postcolumn derivatization with vanillin. The mean recovery of drug was 95% over the 40-320 ng/g range, the coefficient of variation was +/- 10% or better, and no interference was observed from commercial polyether ionophores. The minimum level of detection for semduramicin sodium in broiler liver is 25 ng/g.
本文描述了一种液相色谱（LC）方法，用于在预计使用条件下给药时定量测定肉鸡肝脏中的赛杜霉素钠。在此方法中，用氢氧化铵甲醇溶液从肝脏中提取赛杜霉素钠，通过固相萃取步骤进行分离和浓缩，然后用LC结合香草醛柱后衍生化进行测定。在40 - 320 ng/g范围内，药物的平均回收率为95%，变异系数为±10%或更低，且未观察到来自市售聚醚离子载体的干扰。肉鸡肝脏中赛杜霉素钠的最低检测水平为25 ng/g。
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