Condon K C, Condon G C, Dafa'alla T H, Forrester O T, Phillips C E, Scaife S, Alphey L
Department of Zoology, University of Oxford, South Parks Road, Oxford, UK.
Insect Mol Biol. 2007 Oct;16(5):573-80. doi: 10.1111/j.1365-2583.2007.00752.x.
Germ-line transformation of a major agricultural pest, the Mexican fruit fly (Anastrepha ludens Loew, Mexfly), was achieved using composite piggyBac transposable elements marked with green, yellow and red fluorescent proteins (CopGreen, PhiYFP and J-Red). We also investigated the possibility of generating transposon-free insertions, in order to address potential concerns relating to proposed field use of transgenic Mexfly. We describe a highly efficient method for transforming Mexfly, compare efficiency of piggyBac terminal sequences for transformation and also describe the derivation of a transposon-free insertion line. The development of an efficient transformation system for Mexfly holds great promise for improved applications of the sterile insect technique, a major component of the present control measures for this economically important pest species.
利用标记有绿色、黄色和红色荧光蛋白（CopGreen、PhiYFP和J-Red）的复合piggyBac转座元件，实现了对一种主要农业害虫——墨西哥果蝇（Anastrepha ludens Loew，Mexfly）的种系转化。我们还研究了产生无转座子插入的可能性，以解决与转基因墨西哥果蝇拟在田间使用相关的潜在问题。我们描述了一种高效转化墨西哥果蝇的方法，比较了用于转化的piggyBac末端序列的效率，还描述了一个无转座子插入系的衍生过程。开发一种高效的墨西哥果蝇转化系统，对于改进不育昆虫技术的应用具有巨大潜力，而不育昆虫技术是当前对这种具有经济重要性的害虫物种进行控制的主要措施之一。
