Koukidou M, Klinakis A, Reboulakis C, Zagoraiou L, Tavernarakis N, Livadaras I, Economopoulos A, Savakis C
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, Crete, Greece.
Insect Mol Biol. 2006 Feb;15(1):95-103. doi: 10.1111/j.1365-2583.2006.00613.x.
The olive fruit fly (olive fly) Bactrocera oleae (Dacus), recently introduced in North America, is the most destructive pest of olives worldwide. The lack of an efficient gene transfer technology for olive fly has hampered molecular analysis, as well as development of genetic techniques for its control. We have developed a Minos-based transposon vector carrying a self-activating cassette which overexpresses the enhanced green fluorescent protein (EGFP). Efficient transposase-mediated integration of one to multiple copies of this vector was achieved in the germ line of B. oleae by coinjecting the vector along with in vitro synthesized Minos transposase mRNA into preblastoderm embryos. The self-activating gene construct combined with transposase mRNA present a system with potential for transgenesis of very diverse species.
橄榄实蝇（橄榄果蝇）油橄榄实蝇（原属果实蝇属）最近被引入北美，是全球橄榄最具毁灭性的害虫。缺乏针对橄榄果蝇的高效基因转移技术阻碍了分子分析以及用于其防治的遗传技术的发展。我们开发了一种基于Minos的转座子载体，其携带一个自我激活盒，该盒可过表达增强型绿色荧光蛋白（EGFP）。通过将该载体与体外合成的Minos转座酶mRNA一起注射到胚盘形成前的胚胎中，在油橄榄实蝇的种系中实现了该载体一至多个拷贝的高效转座酶介导的整合。自我激活基因构建体与转座酶mRNA相结合，为非常多样的物种提供了一个具有转基因潜力的系统。
