Zhou Danni, Li Saijiao, Li Wei, Yin Tailang, Xu Wangming, Zhang Jun, Yang Jing
Reproductive Medical Center, Renmin Hospital of Wuhan University, Hubei Clinic Research Center for Assisted Reproductive Technology and Embryonic Development, JieFang Road 238, Wuhan, 430060, PR China.
Obstetrics and Gynecology department, Renmin Hospital of Wuhan University, JieFang Road 238, Wuhan, 430060, China.
Eur J Obstet Gynecol Reprod Biol. 2017 Nov;218:106-112. doi: 10.1016/j.ejogrb.2017.09.017. Epub 2017 Sep 19.
Obesity and low-grade chronic inflammation play critical roles in pathological process of PCOS. PGRN is an adipokine and was recently reported that it could induce a low-grade chronic inflammatory state and plays a functional role in insulin resistance associated with obesity. The overall goal of the present study was to evaluate the levels of PGRN in follicular fluid (FF) and the expression of PGRN in granulosa cells (GCs) with respect to the quality of the oocytes both in patients with PCOS and in the normal ovary during COH cycles.
Ninety-two patients underwent IVF-ET were divided into four groups based on body mass index: normal-weight PCOS group; overweight PCOS group; non-overweight control group and overweight control group. FF samples and GCs were collected at the time of oocyte retrieval. The PGRN, TNF-α, IL-6 and MCP-1 levels were measured by ELISA, and the mRNA expression of PGRN in GCs was detected by real-time polymerase chain reaction.
Analysis of PGRN expression revealed that PGRN levels in FF and the mRNA expression of PGRN in GCs were higher in patients with PCOS than in control patients, and higher in overweight patients than in the normal-weight patients; PGRN in FF of PCOS was positively correlate with basal testosterone and FF TNF-α，but negative correlation with retrieved oocytes number CONCLUSION: This study suggests that PGRN may be a crucial determinant of fertilization success for PCOS patients.
肥胖和低度慢性炎症在多囊卵巢综合征（PCOS）的病理过程中起关键作用。颗粒蛋白前体（PGRN）是一种脂肪因子，最近有报道称它可诱导低度慢性炎症状态，并在与肥胖相关的胰岛素抵抗中发挥作用。本研究的总体目标是评估在控制性卵巢刺激（COH）周期中，多囊卵巢综合征患者和正常卵巢患者卵泡液（FF）中PGRN的水平以及颗粒细胞（GCs）中PGRN的表达与卵母细胞质量的关系。
将92例行体外受精-胚胎移植（IVF-ET）的患者根据体重指数分为四组：正常体重PCOS组；超重PCOS组；非超重对照组和超重对照组。在取卵时收集FF样本和GCs。通过酶联免疫吸附测定（ELISA）法检测PGRN、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和单核细胞趋化蛋白-1（MCP-1）的水平，并通过实时聚合酶链反应检测GCs中PGRN的mRNA表达。
PGRN表达分析显示，PCOS患者FF中PGRN水平和GCs中PGRN的mRNA表达高于对照患者，超重患者高于正常体重患者；PCOS患者FF中的PGRN与基础睾酮和FF中的TNF-α呈正相关，但与获卵数呈负相关。结论：本研究表明，PGRN可能是PCOS患者受精成功的关键决定因素。
