Yamagata Junpei, Ahmed Kamruddin, Khawplod Pakamatz, Mannen Kazuaki, Xuyen Dinh Kim, Loi Huynh Huu, Dung Nguyen Van, Nishizono Akira
Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, Oita University, Yufu, Oita, Japan.
Microbiol Immunol. 2007;51(9):833-40. doi: 10.1111/j.1348-0421.2007.tb03979.x.
The present study was done to determine the molecular epidemiology of rabies virus (RV) in Vietnam. The nucleoprotein (N) and glycoprotein (G) genes of RVs were amplified from the brains of ten rabid dogs of Ho Chi Minh City, Vietnam. The nucleotide sequences of these genes were compared with those of other Asian strains to find the possible relationship among them. Phylogenetic analysis revealed that the Asian N gene segregated into three main branches, namely South-East Asia 1 (SEA 1), South-East Asia 2 (SEA 2) and Indian subcontinent (ISC) genotypes. The SEA 1 genotype comprised RVs from Malaysia, Vietnam and Thailand. The SEA 2 genotype contained strains from the Philippines, and the ISC genotype comprised strains from Sri Lanka and India. Phylogenetically G genes of RVs from Vietnam and Thailand were clustered together. Our study suggests that Vietnamese and Thai RVs are closely related and might have originated from a common ancestor.
本研究旨在确定越南狂犬病病毒（RV）的分子流行病学。从越南胡志明市的10只狂犬的大脑中扩增出RV的核蛋白（N）和糖蛋白（G）基因。将这些基因的核苷酸序列与其他亚洲毒株的序列进行比较，以找出它们之间可能的关系。系统发育分析表明，亚洲N基因分为三个主要分支，即东南亚1型（SEA 1）、东南亚2型（SEA 2）和印度次大陆（ISC）基因型。SEA 1基因型包括来自马来西亚、越南和泰国的RV。SEA 2基因型包含来自菲律宾的毒株，ISC基因型包括来自斯里兰卡和印度的毒株。在系统发育上，来自越南和泰国的RV的G基因聚集在一起。我们的研究表明，越南和泰国的RV密切相关，可能起源于共同的祖先。
