Cheng Huan-Chen, Zhang Feng-Wei, Deng Chang-Yan, Jiang Cao-De, Xiong Yuan-Zhu, Li Feng-E, Lei Ming-Gang
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agriculture University, Wuhan, P. R. China.
Genet Sel Evol. 2007 Sep-Oct;39(5):599-607. doi: 10.1186/1297-9686-39-5-599. Epub 2007 Sep 27.
Although expression and epigenetic differences of imprinted genes have been extensively characterised in man and the mouse, little is known on livestock species. In this study, the polymorphism-based approach was used to detect the imprinting status of NNAT and DIRAS3 genes in five heterozygous pigs (based on SNP) of Large White and Meishan F(1) hybrids. The results show that both genes were paternally expressed in all the tested tissues (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, ovary and pituitary). In addition, the NNAT gene had two transcripts in all tested tissues, which is consistent with its counterpart in man and cattle.
尽管印记基因的表达和表观遗传差异在人类和小鼠中已得到广泛研究，但在家畜物种方面却知之甚少。在本研究中，基于多态性的方法被用于检测五个大白猪和梅山猪F(1)杂种杂合猪（基于单核苷酸多态性）中NNAT和DIRAS3基因的印记状态。结果表明，这两个基因在所有测试组织（心脏、肝脏、脾脏、肺、肾脏、胃、小肠、骨骼肌、脂肪、子宫、卵巢和垂体）中均为父源表达。此外，NNAT基因在所有测试组织中都有两种转录本，这与人类和牛中的对应情况一致。
