小鼠中神经调节蛋白差异甲基化区域（DMRs）的鉴定。
Identification of differentially methylated regions (DMRs) of neuronatin in mice.
作者信息
Xu Yuxin, Liu Zhiquan, Wang Tiedong, Chen Xianju, Deng Jichao, Chen Mao, Li Zhanjun
机构信息
College of Animal Science, Jilin University, 5333#, Xi'an Road, Changchun, 130062 China.
出版信息
Springerplus. 2016 Nov 28;5(1):2018. doi: 10.1186/s40064-016-3721-0. eCollection 2016.
BACKGROUND
Neuronatin () is a paternal-inherited imprinted gene, first discovered in the rat neonatal brain, where it plays vital roles for neuronal growth, brain development, and metabolic regulation. The maternal imprint of has been identified in mice; however, the differentially methylated regions (DMRs) involved in the monoallelic expression of have not yet been investigated.
RESULTS
In this study, we confirmed expression of two isoforms of the (α and β) in the mice brain via quantitative RT-PCR. Additionally, the methylation profile of the CpG island located in the gene locus was determined in the mice liver, brain, sperm, and the MII oocyte via bisulfite sequencing PCR.
CONCLUSION
In summary, we provide the first evidence for tissue- and gamete-specific methylation patterns of CpG3 that are located on exon 1, to be putative DMR of in mice.
背景
神经调节蛋白（Neuronatin，Nnat）是一种父系遗传的印记基因，最初在大鼠新生脑中被发现，它在神经元生长、大脑发育和代谢调节中发挥着至关重要的作用。Nnat的母系印记已在小鼠中得到鉴定；然而，参与Nnat单等位基因表达的差异甲基化区域（DMRs）尚未得到研究。
结果
在本研究中，我们通过定量逆转录聚合酶链反应（qRT-PCR）证实了Nnat的两种异构体（α和β）在小鼠脑中的表达。此外，通过亚硫酸氢盐测序PCR（bisulfite sequencing PCR）确定了位于Nnat基因座的CpG岛在小鼠肝脏、大脑、精子和MII卵母细胞中的甲基化谱。
结论
总之，我们首次提供了位于外显子1上的CpG3的组织和配子特异性甲基化模式的证据，CpG3被认为是小鼠中Nnat的DMR。
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