Alves Luís, Marques Susana, Matos José, Tenreiro Rogério, Gírio Francisco M
INETI, Departamento de Biotecnologia, Estrada do Paço do Lumiar, 22, 1649-038 Lisboa, Portugal.
Chemosphere. 2008 Jan;70(6):967-73. doi: 10.1016/j.chemosphere.2007.08.016. Epub 2007 Sep 25.
Enzymatic hydrolyzates of recycled paper sludge were tested as suitable feedstock for biological desulfurization by Gordonia alkanivorans strain 1B. Only the hydrolyzate obtained after enzymatic mixture dialysis (dialyzed hydrolyzate) allowed dibenzothiophene (DBT) desulfurization, in spite of faster bacterial growth did occur on non-dialyzed hydrolyzate. For dialyzed hydrolyzate, 250microM DBT was consumed after 96h displaying a maximum specific productivity of 2-hydroxybiphenyl of 1.1micromol g(-1)(dry cell weight) h(-1). A comparison of the kinetics of biodesulfurization was assessed according to the type of hydrolyzate supplementation. Complete consumption of DBT was observed upon the addition of only phosphates and ammonia although further addition of zinc did increase the 2-hydroxybiphenyl production by 14%. Strain 1B was able to desulfurize a model oil containing DBT, 4-methylDBT and 4,6-dimethylDBT, reducing by 63% the total sulfur content in 168h.
对回收纸污泥的酶水解产物进行了测试，以确定其是否适合作为食烷戈登氏菌1B菌株进行生物脱硫的原料。尽管在未透析的水解产物上细菌生长更快，但只有经过酶混合物透析后得到的水解产物（透析水解产物）才能实现二苯并噻吩（DBT）脱硫。对于透析水解产物，96小时后消耗了250μM DBT，2-羟基联苯的最大比生产率为1.1μmol g⁻¹（干细胞重量）h⁻¹。根据水解产物补充类型评估了生物脱硫动力学的比较。仅添加磷酸盐和氨时观察到DBT完全消耗，尽管进一步添加锌确实使2-羟基联苯产量提高了14%。1B菌株能够对含有DBT、4-甲基DBT和4,6-二甲基DBT的模型油进行脱硫，在168小时内将总硫含量降低了63%。
