Cavalcanti B C, Moura D J, Rosa R M, Moraes M O, Araujo E C C, Lima M A S, Silveira E R, Saffi J, Henriques J A P, Pessoa C, Costa-Lotufo L V
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Rua Cel Nunes de Melo, 1127, Caixa Postal-3157, 60430-270 Fortaleza, Ceará, Brazil.
Food Chem Toxicol. 2008 Jan;46(1):388-92. doi: 10.1016/j.fct.2007.08.009. Epub 2007 Aug 19.
The genotoxic effect of two tanshinones isolated from roots of Hyptis martiussi Benth (Labiatae) was studied using V79 (Chinese hamster lung) cells by the alkaline comet assay and micronucleus test. Tanshinones were incubated with the cells at concentrations of 1, 3, 6 and 12 microg/mL for 3 h. Tanshinones were shown to be quite strongly genotoxic against V79 cells at all tested concentrations. The data obtained provide support to the view that tanshinones has DNA damaging activity in cultured V79 cells under the conditions of the assays.
利用碱性彗星试验和微核试验，以V79（中国仓鼠肺）细胞研究了从唇形科植物马特休斯香科科（Hyptis martiussi Benth）根部分离得到的两种丹参酮的遗传毒性作用。将丹参酮与细胞在浓度为1、3、6和12μg/mL的条件下孵育3小时。结果表明，在所有测试浓度下，丹参酮对V79细胞均具有较强的遗传毒性。所获得的数据支持了在试验条件下丹参酮在培养的V79细胞中具有DNA损伤活性这一观点。
