de Oliveira Allan Demetrius Leite, Galvao Rodrigue Fabiola Fernandes, Douglas Melo Coutinho Henrique, da Costa Jose Galberto Martins, de Menezes Irwin Rose Alencar
Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry, Regional University of Cariri-URCA, Crato-CE, Brazil ; Faculty of Leao Sampaio, Lagoa Seca Campus, Juazeiro do Norte-CE, Brazil.
Department of Biological Chemistry, Laboratory of Natural Product Research, Regional University of Cariri-URCA, Crato-CE, Brazil.
Jundishapur J Nat Pharm Prod. 2014 Jun 29;9(3):e13521. doi: 10.17795/jjnpp-13521. eCollection 2014 Aug.
Several studies have shown that species of the genus Hyptis, have promising antimicrobial and antifungal effects.
Identify of chemical constituents of essential oil from leaves of Hyptis martiusii and evaluate its effect against bacterial strains by direct and gaseous contact.
Essential oil was extracted from leaves of Hyptis martiusii Benth using hydro-distillation, and its composition was determined using gas chromatography-mass spectrometry (GC-MS). Chemical analysis showed that there was a predominance of sesquiterpenes. The leaf essential oil was screened for its minimal inhibitory concentration and modulatory effect of aminoglycoside by the direct (MIC) and gaseous (MID) micro-dilution assays for various pathogenic microorganisms. The essential oil remarkably inhibited the growth of all of the tested bacteria (MIC < 512 μg/mL) except S. aureus (SA358) multidrug resistant (MRSA) by direct contact.
Twenty-four compounds representing 92.13% of the essential oil of leaves were characterized; δ -3-carene (6.88%), 1, 8-cineole (7.01%), trans-caryophyllene (9.21%), Cariophyllene oxide (7.47%) and bicyclogermacrene (10.61%) were found as the major components. Modulatory aminoglycoside effect, by direct contact, was showed antagonistic relationship with antimicrobial activity. The gaseous component of the oil inhibited the bacterial growth of all of the tested bacteria in 50% and 25% of oil concentration and demonstrated synergistic interactions can be attributed to the constituting the oil compounds.
These results show that this oil influences the activity of the antibiotic and may be used as an adjuvant in the antibiotic therapy of respiratory tract bacterial pathogens.
多项研究表明，山香属植物具有良好的抗菌和抗真菌作用。
鉴定马蒂乌斯山香叶片精油的化学成分，并通过直接接触和气态接触评估其对细菌菌株的作用。
采用水蒸馏法从马蒂乌斯山香（Hyptis martiusii Benth）叶片中提取精油，并用气相色谱 - 质谱联用仪（GC - MS）测定其成分。化学分析表明倍半萜类化合物占主导地位。通过直接（MIC）和气态（MID）微量稀释法对各种致病微生物进行检测，以筛选叶片精油的最低抑菌浓度及其对氨基糖苷类药物的调节作用。通过直接接触，该精油能显著抑制除耐多药金黄色葡萄球菌（SA358）之外的所有受试细菌的生长（MIC < 512 μg/mL）。
鉴定出占叶片精油92.13%的24种化合物；主要成分包括δ - 3 - 蒈烯（6.88%）、1, 8 - 桉叶素（7.01%）、反式石竹烯（9.21%）、石竹烯氧化物（7.47%）和双环大根香叶烯（10.61%）。通过直接接触，调节氨基糖苷类药物的作用与抗菌活性呈拮抗关系。该精油的气态成分在50%和25%的油浓度下能抑制所有受试细菌的生长，并证明协同相互作用可归因于构成该精油的化合物。
这些结果表明该精油会影响抗生素的活性，可作为呼吸道细菌病原体抗生素治疗的佐剂。
