Torres-Carrillo Nora Magdalena, Torres-Carrillo Norma, Vázquez-Del Mercado Mónica, Delgado-Rizo Vidal, Oregón-Romero Edith, Parra-Rojas Isela, Muñoz-Valle José Francisco
Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Col. Independencia, Guadalajara, Jalisco 44340, Mexico.
Rheumatol Int. 2008 Feb;28(4):355-60. doi: 10.1007/s00296-007-0453-z. Epub 2007 Sep 26.
We assessed whether the -844 G/A polymorphism and mRNA expression of plasminogen activator inhibitor 1 (PAI-1) gene are associated with rheumatoid arthritis (RA). Demographic data, hematological, biochemical parameters, disease activity-disability indexes, -844 G/A genotypes and mRNA expression levels of the PAI-1 gene were determined in 50 RA patients and 50 healthy subjects (HS). Non-significant differences in genotype and allele frequencies related to -844 G/A polymorphism in RA versus HS, were found. High mRNA expression of the PAI-1 gene, was demonstrated in RA versus HS (P < 0.05). In addition, A/A genotype carriers showed increase of PAI-1 mRNA expression (3.1-fold) respect to G/G and G/A genotypes in RA patients (P < 0.05). Our finding suggest an association of A/A -844 PAI-1 genotype with high PAI-1 mRNA expression in RA patients.
我们评估了纤溶酶原激活物抑制剂1（PAI-1）基因的-844 G/A多态性和mRNA表达是否与类风湿性关节炎（RA）相关。测定了50例RA患者和50例健康对照者（HS）的人口统计学数据、血液学和生化参数、疾病活动-残疾指数、-844 G/A基因型以及PAI-1基因的mRNA表达水平。结果发现，RA患者与HS在-844 G/A多态性的基因型和等位基因频率上无显著差异。与HS相比，RA患者中PAI-1基因的mRNA高表达（P < 0.05）。此外，在RA患者中，A/A基因型携带者的PAI-1 mRNA表达相对于G/G和G/A基因型增加了3.1倍（P < 0.05）。我们的研究结果表明，RA患者中A/A -844 PAI-1基因型与PAI-1 mRNA高表达相关。
