Xu Xia, Wang Hongchao, Li Haijun, Cui Xiaopei, Zhang Hongyu
1 Department of Geriatrics, Qilu Hospital, Shandong University, Jinan, China.
2 Department of Pulmonary Medicine, Jinan Central Hospital, Shandong University, Jinan, China.
J Int Med Res. 2016 Dec;44(6):1292-1301. doi: 10.1177/0300060516664270. Epub 2016 Nov 18.
Objective To investigate the association between serpin family E member 1 ( SERPINE1) -844 A/G and -675 4G/5G polymorphisms and chronic obstructive pulmonary disease (COPD) in a Chinese Han population. Method SERPINE1 -844 A/G and -675 4G/5G polymorphisms were assessed by polymerase chain reaction-restriction fragment length polymorphism sequencing of genomic DNA from patients with COPD and healthy smoking controls. Results Out of 140 patients with COPD and 100 controls, all SERPINE1 -844 and -675 polymorphisms were in Hardy-Weinberg equilibrium. Differences in SERPINE1 -675 4G and 5G allele frequencies were statistically significant between the COPD and control groups (odds ratio [OR] 1.45, 95% confidence interval [CI] 1.00, 2.09), but there was no significant between-group difference in SERPINE1 -844 A and G allele frequencies. The SERPINE1 -675 4G/4G genotype was associated with COPD (OR 1.87, 95% CI 1.06, 3.32 [binary logistic regression]). Haplotype analysis showed that COPD was associated with SERPINE1 -844G/4G (OR 2.11, 95% CI 1.32, 3.38) and SERPINE1 -844G/5G (OR 0.66, 95% CI 0.45, 0.95). Conclusion The SERPINE1 -675 polymorphism, but not SERPINE1 -844 polymorphism, was associated with susceptibility to COPD in a Chinese Han population.
目的 探讨丝氨酸蛋白酶抑制剂E1家族成员1（SERPINE1）-844 A/G和-675 4G/5G多态性与中国汉族人群慢性阻塞性肺疾病（COPD）的相关性。方法 采用聚合酶链反应-限制性片段长度多态性测序技术，对COPD患者和健康吸烟对照者的基因组DNA进行SERPINE1 -844 A/G和-675 4G/5G多态性检测。结果 在140例COPD患者和100例对照者中，所有SERPINE1 -844和-675多态性均处于Hardy-Weinberg平衡。COPD组和对照组之间SERPINE1 -675 4G和5G等位基因频率差异有统计学意义（优势比[OR] 1.45，95%置信区间[CI] 1.00，2.09），但SERPINE1 -844 A和G等位基因频率在两组间无显著差异。SERPINE1 -675 4G/4G基因型与COPD相关（OR 1.87，95% CI 1.06，3.32[二元逻辑回归]）。单倍型分析显示，COPD与SERPINE1 -844G/4G（OR 2.11，95% CI 1.32，3.38）和SERPINE1 -844G/5G（OR 0.66，95% CI 0.45，0.95）有关。结论 SERPINE1 -675多态性而非SERPINE1 -844多态性与中国汉族人群COPD易感性相关。
