Bhattacharya Sudhindra Mohan
Department of Obstetrics and Gynecology, Vivekananda Institute of Medical Sciences, 99, Sarat Bose Road, Kolkata 700026, India.
Int Urol Nephrol. 2008;40(2):391-5. doi: 10.1007/s11255-007-9282-y.
To study the relationship of various sperm characteristics with repeated unexplained early pregnancy loss.
Semen samples from husbands of 74 couples with the history of repeated early pregnancy loss were analyzed as per WHO criteria, and DNA integrity was studied in each case by Acridine Orange staining test. Semen samples from 65 husbands of proven fertility were also studied for comparison.
No statistically significant differences were noted in the age of the husbands, sperm concentration, total count per ejaculate, and rapid progressive motility of sperms between the two groups. On the other hand, significant differences were found in total motile sperms per ejaculate, percentage of motile sperm and, most importantly, in the DNA integrity value.
The sperm DNA-integrity damage is an important paternal factor associated with repeated embryonic or early fetal loss and should be included in the evaluation of these couples.
研究各种精子特征与反复不明原因早期妊娠丢失之间的关系。
按照世界卫生组织标准对74对有反复早期妊娠丢失病史夫妇的丈夫的精液样本进行分析，并通过吖啶橙染色试验研究每例样本的DNA完整性。还对65名经证实有生育能力的丈夫的精液样本进行研究以作比较。
两组丈夫的年龄、精子浓度、每次射精的总数以及精子的快速前向运动能力方面未发现统计学上的显著差异。另一方面，在每次射精的总活动精子数、活动精子百分比，以及最重要的是在DNA完整性值方面发现了显著差异。
精子DNA完整性损伤是与反复胚胎或早期胎儿丢失相关的一个重要父方因素，应纳入对这些夫妇的评估中。
