Borini A, Tarozzi N, Bizzaro D, Bonu M A, Fava L, Flamigni C, Coticchio G
Tecnobios Procreazione, Centre for Reproductive Health, Via Dante 15, Bologna, Italy.
Hum Reprod. 2006 Nov;21(11):2876-81. doi: 10.1093/humrep/del251. Epub 2006 Jun 22.
The relationship between early embryo post-implantation development in couples undergoing assisted reproductive techniques (ARTs) and sperm chromatin alterations has not been satisfactorily explained. The aim of this study was to assess the relationship between sperm DNA fragmentation in IVF/ICSI patients, sperm parameters (concentration, motility and morphology) and ART outcome, especially with regard to clinical pregnancy and pregnancy loss (spontaneous miscarriage or biochemical pregnancy).
DNA fragmentation was evaluated by TUNEL assay, performed on sperm suspensions after density gradient separation, in 132 men undergoing an ART cycle (82 IVF and 50 ICSI) and correlated with sperm parameters and ART outcome.
A highly significant negative correlation was found between DNA fragmentation and sperm parameters. There was a close relationship between DNA fragmentation and post-implantation development in ICSI patients: the clinical pregnancy and pregnancy loss rates significantly differed between patients with high and low sperm DNA fragmentation (P = 0.007 and P = 0.009, respectively).
Sperm DNA fragmentation seems to affect embryo post-implantation development in ICSI procedures: high sperm DNA fragmentation can compromise 'embryo viability', resulting in pregnancy loss.
辅助生殖技术（ART）夫妇中早期胚胎着床后发育与精子染色质改变之间的关系尚未得到令人满意的解释。本研究的目的是评估体外受精/卵胞浆内单精子注射（IVF/ICSI）患者的精子DNA片段化、精子参数（浓度、活力和形态）与ART结局之间的关系，尤其是关于临床妊娠和妊娠丢失（自然流产或生化妊娠）。
对132名接受ART周期治疗的男性（82例IVF和50例ICSI），在密度梯度分离后的精子悬液上进行末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）测定来评估DNA片段化，并将其与精子参数和ART结局相关联。
发现DNA片段化与精子参数之间存在高度显著的负相关。在ICSI患者中，DNA片段化与着床后发育之间存在密切关系：精子DNA片段化高和低的患者之间临床妊娠率和妊娠丢失率有显著差异（分别为P = 0.007和P = 0.009）。
在ICSI操作中，精子DNA片段化似乎会影响胚胎着床后发育：高精子DNA片段化会损害“胚胎活力”，导致妊娠丢失。
