精子染色质扩散试验在不明原因复发性流产夫妇中的价值。
Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion.
机构信息
Department of Anatomical sciences, Ahvaz Jundishapor University of Medical Science, P.O. Box: 61357-15794, Ahvaz, Iran,
出版信息
J Assist Reprod Genet. 2012 Jan;29(1):11-4. doi: 10.1007/s10815-011-9647-0. Epub 2011 Oct 19.
OBJECTIVE
To evaluate and compare standard sperm parameters and sperm chromatin integrity by sperm chromatin dispersion test (SCD) in ejaculates from men whose partners have a history of recurrent pregnancy loss and from control group of fertile men.
METHODS
Thirty couples with unexplained recurrent abortion (case group) and 30 fertile couples (control group) referring to Shiraz infertility center were included. Sperm parameters were assessed in semen samples from two groups and then staining with SCD procedure. The results were analyzed by performing ANOVA and Tukey(,)s tests.
RESULTS
In control group, nucleoids with big (65.93 ± 2.35), small (12.4 ± 0.60) and without halo (11.6 ± 0.50) showed significant difference with case group (41.40 ± 1.43), (21.16 ± 1.11) and (23.26 ± 1.10) respectively. In the RPL group spermatozoa with high percentage of abnormal parameters (morphology and motility) was observed (p ≤ 0.05).
CONCLUSION
This study strengthens the current literature associating sperm quality with recurrent pregnancy loss, and emphasizes the important of evaluating male factor by tests such as SCD in addition to conventional sperm parameters.
目的
评估并比较有反复妊娠丢失史男性和生育力正常男性精液中的标准精子参数和精子染色质完整性，精子染色质扩散试验（SCD）。
方法
将 30 对不明原因反复流产的夫妇（病例组）和 30 对生育力正常的夫妇（对照组）纳入 Shiraz 不孕不育中心。对两组精液样本的精子参数进行评估，然后用 SCD 程序进行染色。采用 ANOVA 和 Tukey（，）检验对结果进行分析。
结果
对照组中，大核（65.93±2.35）、小核（12.4±0.60）和无晕核（11.6±0.50）的核小体与病例组（41.40±1.43）、（21.16±1.11）和（23.26±1.10）相比有显著差异。在 RPL 组中，观察到具有高比例异常参数（形态和活力）的精子（p≤0.05）。
结论
本研究加强了精子质量与反复妊娠丢失相关的现有文献，并强调了除常规精子参数外，通过 SCD 等测试评估男性因素的重要性。
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