Cauwerts K, Decostere A, De Graef E M, Haesebrouck F, Pasmans F
Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, Belgium.
Avian Pathol. 2007 Oct;36(5):395-9. doi: 10.1080/03079450701589167.
A total of 73 isolates of Enterococcus spp. carrying the erm(B) gene were obtained from cloacal swabs of broiler chickens derived from 13 different farms in Belgium. The erm(B) gene encodes resistance to macrolides, lincosamides and streptogramin B antibiotics (MLS(B)). The isolates belonged to eight different species: Enterococcus avium (eight isolates), Enterococcus casseliflavus (11 isolates), Enterococcus cecorum (eight isolates), Enterococcus durans (seven isolates), Enterococcus faecalis (10 isolates), Enterococcus faecium (17 isolates), Enterococcus gallinarum (seven isolates) and Enterococcus hirae (five isolates). Acquired resistance to tetracycline was detected in 68 of the isolates, and in 62 of these it was associated with the presence of the resistance genes tet(L), tet(M), tet(O) or tet(S). In three E. faecium isolates that were phenotypically susceptible to tetracycline, tet(L) or tet(M) was present. The transposon integrase gene (int gene) of the Tn916/Tn1545 transposon family was detected in 18 of the 54 isolates that contained the tet(M) gene. It was concluded that acquired resistance to tetracycline antibiotics is often present in enterococci from poultry carrying the erm(B) gene. The use of tetracyclines in poultry may therefore co-select for resistance to MLS(B) antibiotics, which may be important as alternative therapy for enterococcal infections in humans.
从比利时13个不同农场的肉鸡泄殖腔拭子中总共获得了73株携带erm(B)基因的肠球菌分离株。erm(B)基因编码对大环内酯类、林可酰胺类和链阳菌素B类抗生素（MLS(B)）的抗性。这些分离株属于八个不同的种：鸟肠球菌（8株）、格氏肠球菌（11株）、盲肠肠球菌（8株）、耐久肠球菌（7株）、粪肠球菌（10株）、屎肠球菌（17株）、鹑鸡肠球菌（7株）和平肠球菌（5株）。在68株分离株中检测到对四环素的获得性抗性，其中62株与抗性基因tet(L)、tet(M)、tet(O)或tet(S)的存在有关。在3株表型上对四环素敏感的屎肠球菌分离株中，存在tet(L)或tet(M)。在含有tet(M)基因的54株分离株中的18株中检测到Tn916/Tn1545转座子家族的转座子整合酶基因（int基因）。得出的结论是，携带erm(B)基因的家禽肠球菌中常常存在对四环素类抗生素的获得性抗性。因此，在家禽中使用四环素类药物可能会共同选择对MLS(B)抗生素的抗性，这对于人类肠球菌感染的替代治疗可能很重要。
