Ojkic Davor, Martin Emily, Swinton Janet, Binnington Brian, Brash Marina
Animal Health Laboratory, University of Guelph, Ontario, Guelph, Canada.
Avian Pathol. 2007 Oct;36(5):427-33. doi: 10.1080/03079450701598408.
For this retrospective study, infectious bursal disease virus (IBDV) was detected in 134 bursal samples that originated from flocks with conditions such as airsacculitis, tracheitis, pneumonia, septicaemia, inclusion body hepatitis, coccidiosis, and/or a history of production problems without overt clinical symptoms. Samples were from seven Canadian provinces: Ontario, Quebec, Manitoba, British Columbia, Nova Scotia, Alberta, and Newfoundland and Labrador. Viral RNA was identified in bursae with moderate to severe and acute to chronic bursal damage. The ages of the flocks from which samples were collected ranged from 3 to 63 days. Following reverse transcriptase-polymerase chain reaction the nucleotide sequence of the VP2 hypervariable region was determined and compared with sequences available in GenBank. The most common Canadian IBDV field strains were North-American variant viruses. Forty-four viruses were highly related (97.5% to 100.0%) to the US IBDV strain NC171. Moreover, 16 field viruses whose VP2 sequences were 99.2% to 100% identical to the South African 05SA8 IBDV strain appeared closely related to the NC171 group. Delaware E-related field viruses, 98.3% to 100.0% identical to the prototype virus, were identified in 33 samples. Thirty-four Canadian IBDVs showed the highest identity, 94.2% to 98.3%, to US IBDV strain 586. Five samples contained vaccine-related viruses, while two field strains showed the best match to Del A (United States) and IBDV strains SP_04_02 (Spain). Very virulent IBDVs were not detected in Canada.
在这项回顾性研究中，从患有气囊炎、气管炎、肺炎、败血症、包涵体肝炎、球虫病和/或有生产问题病史但无明显临床症状的鸡群采集的134份法氏囊样本中检测到传染性法氏囊病病毒（IBDV）。样本来自加拿大的七个省份：安大略省、魁北克省、马尼托巴省、不列颠哥伦比亚省、新斯科舍省、艾伯塔省以及纽芬兰和拉布拉多省。在法氏囊有中度至重度以及急性至慢性损伤的样本中鉴定出病毒RNA。采集样本的鸡群年龄范围为3至63日龄。经过逆转录-聚合酶链反应后，确定了VP2高变区的核苷酸序列，并与GenBank中可用的序列进行了比较。加拿大最常见的IBDV田间毒株是北美变异病毒。44种病毒与美国IBDV毒株NC171高度相关（97.5%至100.0%）。此外，16种田间病毒的VP2序列与南非05SA8 IBDV毒株的序列一致性为99.2%至100%，它们似乎与NC171组密切相关。在33份样本中鉴定出与原型病毒一致性为98.3%至100.0%的特拉华E相关田间病毒。34种加拿大IBDV与美国IBDV毒株586的一致性最高，为94.2%至98.3%。5份样本含有与疫苗相关的病毒，而两种田间毒株与Del A（美国）和IBDV毒株SP_04_02（西班牙）的匹配度最佳。在加拿大未检测到超强毒IBDV。
