Srinivas Mangala, Morel Penelope A, Ernst Lauren A, Laidlaw David H, Ahrens Eric T
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.
Magn Reson Med. 2007 Oct;58(4):725-34. doi: 10.1002/mrm.21352.
This article describes an in vivo imaging method for visualizing and quantifying a specific cell population. Cells are labeled ex vivo with a perfluoropolyether nanoparticle tracer agent and then detected in vivo using (19)F MRI following cell transfer. (19)F MRI selectively visualizes only the labeled cells with no background, and a conventional (1)H image taken in the same imaging session provides anatomical context. Using the nonobese diabetic mouse, an established model of type 1 diabetes, (19)F MRI data were acquired showing the early homing behavior of diabetogenic T cells to the pancreas. A computational algorithm provided T cell counts in the pancreas. Approximately 2% of the transferred cells homed to the pancreas after 48 hr. The technique allows for both unambiguous detection of labeled cells and quantification directly from the in vivo images. The in vivo quantification and cell trafficking patterns were verified using (19)F spectroscopy and fluorescence microscopy in excised pancreata. The labeling procedure did not affect T-cell migration in vivo. This imaging platform is applicable to many cell types and disease models and can potentially be used for monitoring the trafficking of cellular therapeutics.
本文描述了一种用于可视化和定量特定细胞群体的体内成像方法。细胞在体外用全氟聚醚纳米颗粒示踪剂进行标记，然后在细胞转移后使用（19）F磁共振成像在体内进行检测。（19）F磁共振成像仅选择性地可视化标记的细胞而无背景干扰，并且在同一成像过程中拍摄的传统（1）H图像提供解剖学背景。使用非肥胖糖尿病小鼠（一种已建立的1型糖尿病模型），获取了（19）F磁共振成像数据，显示致糖尿病T细胞向胰腺的早期归巢行为。一种计算算法提供了胰腺中的T细胞计数。48小时后，约2%的转移细胞归巢至胰腺。该技术既能明确检测标记细胞，又能直接从体内图像进行定量。使用（19）F光谱和荧光显微镜对切除的胰腺进行检测，验证了体内定量和细胞迁移模式。标记过程不影响T细胞在体内的迁移。这种成像平台适用于多种细胞类型和疾病模型，并有可能用于监测细胞治疗药物的运输。
