Van Zeeland A A, Simons J W
Mutat Res. 1976 Apr;35(1):129-37. doi: 10.1016/0027-5107(76)90175-5.
The expression time for induced mutants resistant to 6-thioguanine, in V-79 Chinese hamster cells, was determined by respreading the cells in the selective medium, at various times after treatment. The length of the expression time for mutants induced by X-rays, ethyl methane sulphonate and ultraviolet irradiation was dose dependent. For the highest dose used this was 7 to 8 days, beyond which there was no further changes in mutant frequency. The dose-response relationship of these agents does not appear to deviate from linearity; this permits the calculation of mutation rate per unit dose. For X-rays this value was 1.35 - 10(-7) per rad per locus, for ethyl methane sulphonate, 2.2 - 10(-2) per mole per locus and for ultraviolet irradiation, 6.3 - 10(-6) per erg per mm2 per locus. The effectiveness of the 3 different mutagens for the induction of mutations was compared by calculating the increase in mutant frequency per unit of decrease in survival (Do). These increments in frequency were: 5.6 - 10(-5) for X-rays, 69.5 - 10(-5) for ethyl methane sulphonate and 16.1 - 10(-5) for ultraviolet irradiation.
通过在处理后的不同时间，将V - 79中国仓鼠细胞在选择培养基中再次铺板，测定了对6 - 硫鸟嘌呤有抗性的诱导突变体的表达时间。X射线、甲基磺酸乙酯和紫外线照射诱导的突变体的表达时间长度与剂量相关。对于所使用的最高剂量，表达时间为7至8天，超过这个时间后突变频率不再有进一步变化。这些诱变剂的剂量 - 反应关系似乎不偏离线性；这允许计算每单位剂量的突变率。对于X射线，这个值是每拉德每基因座1.35×10⁻⁷，对于甲基磺酸乙酯，是每摩尔每基因座2.2×10⁻²，对于紫外线照射，是每尔格每平方毫米每基因座6.3×10⁻⁶。通过计算每单位存活降低量（Do）中突变频率的增加，比较了这3种不同诱变剂诱导突变的有效性。这些频率增量分别为：X射线为5.6×10⁻⁵，甲基磺酸乙酯为69.5×10⁻⁵，紫外线照射为16.1×10⁻⁵。
