Wu Jiaqi, Wu Yuanying, Jiang Yiwei, Li Hongzhuan, Zhang Xiaogang, Yang Tianfu
Department of Orthopaedics, West China Hospital of Sichuan University, Chengdu 610041, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2007 Aug;24(4):889-93.
This study was designed to investigate the effects of some Traditional Chinese Medicine (TCM) agents on bone resorption and morphometric features of osteoclasts as well as their relationships. TCM ShengGuZaiZaoSan and XianLingGuBao, were used to treat the experimental fracture. Thirty 6-month-old Chinchilla rabbits were used for the establishment of animal models each with a 3 mm bone defect in the middle of left radius as well as of right radius. These models were divided randomly into 3 groups : ShengGuZaiZaoSan Group (Group A), XianLingGuBao groups (Group B) and control-group (Group C). Every group was further divided into 2 subgroups: a former sacrificed group (14 days after operation) and a latter sacrificed group (31 days after operation). After the rabbits being killed, the samples of their undecalcified calli were subjected to the morphometry study of bone resorption and osteoclasts. Group A had more bone resorption, compared with Group B and C. Both Groups A and B exhibited some changed morphometric features of osteoclasts as compared with Group C (P < 0.05). Simple correlation analysis indicated that bone resorption is mainly correlated with osteoclast numbers, and that in individual group, bone resorption is correlated with osteoclast form factor, area and mean photodensity (P < 0.05). These allow us to conclude that ShengGuZaiZaoSan can increase bone resorption and accelerate bone remodeling by increasing osteoclast numbers at the former stage and can enhance osteoclast function at the latter stage. These changes are beneficial to fracture healing.
本研究旨在探讨某些中药制剂对破骨细胞骨吸收及形态计量学特征的影响及其相互关系。采用中药生骨再造散和仙灵骨葆治疗实验性骨折。选用30只6月龄青紫蓝兔建立动物模型，在左、右桡骨中段造成3mm骨缺损。将这些模型随机分为3组：生骨再造散组（A组）、仙灵骨葆组（B组）和对照组（C组）。每组再进一步分为2个亚组：早期处死组（术后14天）和晚期处死组（术后31天）。兔处死后，对其未脱钙骨痂样本进行骨吸收和破骨细胞的形态计量学研究。与B组和C组相比，A组有更多的骨吸收。与C组相比，A组和B组破骨细胞的形态计量学特征均有一些改变（P<0.05）。简单相关分析表明，骨吸收主要与破骨细胞数量相关，在各个组中，骨吸收与破骨细胞形态因子、面积和平均光密度相关（P<0.05）。由此我们得出结论，生骨再造散在前一阶段可通过增加破骨细胞数量来增加骨吸收并加速骨重塑，在后一阶段可增强破骨细胞功能。这些变化有利于骨折愈合。
