基质辅助激光解吸电离成像质谱：生化系统的分子快照

MALDI imaging mass spectrometry: molecular snapshots of biochemical systems.

作者信息

Cornett Dale S, Reyzer Michelle L, Chaurand Pierre, Caprioli Richard M

机构信息

Mass Spectrometry Research Center, 465 21st Avenue, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA.

出版信息

Nat Methods. 2007 Oct;4(10):828-33. doi: 10.1038/nmeth1094.

DOI:10.1038/nmeth1094

PMID:17901873

Abstract

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is emerging as a powerful tool for investigating the distribution of molecules within biological systems through the direct analysis of thin tissue sections. Unique among imaging methods, MALDI-IMS can determine the distribution of hundreds of unknown compounds in a single measurement. We discuss the current state of the art of MALDI-IMS along with some recent applications and technological developments that illustrate not only its current capabilities but also the future potential of the technique to provide a better understanding of the underlying molecular mechanisms of biological processes.

摘要

基质辅助激光解吸/电离（MALDI）成像质谱（IMS）正逐渐成为一种强大的工具，可通过对薄组织切片的直接分析来研究生物系统中分子的分布。在成像方法中，MALDI-IMS独具特色，能够在一次测量中确定数百种未知化合物的分布。我们将讨论MALDI-IMS的当前技术水平，以及一些近期的应用和技术发展，这些不仅展示了其当前的能力，还体现了该技术在更好地理解生物过程潜在分子机制方面的未来潜力。