Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
J Proteome Res. 2010 Feb 5;9(2):1157-64. doi: 10.1021/pr900666q.
The specific molecular profiles of ovarian cancer interface zones (IZ), the region between tumors and normal tissues, were evaluated using a new method involving matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). We analyzed three ovarian serous carcinomas using MALDI-IMS. Principal component analysis (PCA) was used to evaluate the quality of tissue spatial features based on MALDI-IMS, and for analysis of large data sets of MALDI-IMS. Two-dimensional gel electrophoresis and fluorescence microscopy were used to verify interface-specific proteins. Unique profiles were identified for the tumors, the normal zone, and the IZ. Through MALDI analysis, two interface-specific proteins, plastin 2 and peroxiredoxin 1 (PRDX 1), were identified as differentially regulated between zones. Fluorescence microscopy revealed high expression levels of plastin 2 and PRDX 1 along the IZ of ovarian tumors. This comparative proteomics study using tissue MALDI-IMS suggested that the IZ is different from the adjacent tumor and normal zones, and that plastin 2 and PRDX 1 may be interface markers specific to ovarian tumors.
采用基质辅助激光解吸/电离（MALDI）-成像质谱（IMS）新技术，评估卵巢癌交界区（IZ），即肿瘤与正常组织之间区域的特定分子特征。我们使用 MALDI-IMS 分析了 3 例卵巢浆液性癌。主成分分析（PCA）用于评估基于 MALDI-IMS 的组织空间特征的质量，以及用于分析 MALDI-IMS 的大数据集。二维凝胶电泳和荧光显微镜用于验证交界区特异性蛋白。鉴定到肿瘤、正常区和交界区具有独特的特征谱。通过 MALDI 分析，鉴定到 2 种交界区特异性蛋白，即肌动蛋白结合蛋白 2 和过氧化物还原酶 1（PRDX 1），在区之间呈现差异性调节。荧光显微镜显示，在卵巢肿瘤的交界区，肌动蛋白结合蛋白 2 和 PRDX 1 的表达水平较高。这项应用组织 MALDI-IMS 的比较蛋白质组学研究表明，交界区与相邻肿瘤和正常组织不同，肌动蛋白结合蛋白 2 和 PRDX 1 可能是卵巢肿瘤的特异性交界区标志物。
