Dalpathado Dilusha S, Chang Qing, Burkett Colleen M, Bandu Mary L, Desaire Heather
Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA.
Rapid Commun Mass Spectrom. 2007;21(20):3365-72. doi: 10.1002/rcm.3229.
Recently, we demonstrated a new method, STEP (Statistical Test of Equivalent Pathways) analysis, which differentiates first-generation product ions (primary product ions) from second-generation product ions (secondary product ions) obtained in tandem mass spectrometric (MS/MS) experiments on a quadrupole ion trap mass spectrometer. The study presented here defines how to adapt the STEP method to a more routinely used mass analyzer, the triple quadrupole. New ion activation conditions were developed to adapt the STEP method to the triple quadrupole mass spectrometer using peptides and carbohydrates. The application of this method to the triple quadrupole is useful because it provides an efficient approach to differentiate primary and secondary ions on this instrument. Out of the total number of ions that were subjected to the STEP analysis, this method correctly identified 96% of ions as primary or secondary, indicating that this analysis is effective for carbohydrates and peptides undergoing collision-induced dissociation (CID) on a triple quadrupole mass spectrometer.
最近，我们展示了一种新方法——等效途径统计测试（STEP）分析，该方法可区分在四极杆离子阱质谱仪上进行的串联质谱（MS/MS）实验中获得的第一代产物离子（初级产物离子）和第二代产物离子（次级产物离子）。本文介绍的研究定义了如何将STEP方法应用于更常用的质量分析器——三重四极杆。开发了新的离子活化条件，以便使用肽和碳水化合物将STEP方法应用于三重四极杆质谱仪。该方法在三重四极杆上的应用很有用，因为它提供了一种在该仪器上区分初级和次级离子的有效方法。在接受STEP分析的离子总数中，该方法正确识别出96%的离子为初级或次级离子，这表明该分析对于在三重四极杆质谱仪上经历碰撞诱导解离（CID）的碳水化合物和肽是有效的。
