Segarra Guillem, Jáuregui Olga, Casanova Eva, Trillas Isabel
Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Catalonia, Spain.
Phytochemistry. 2006 Feb;67(4):395-401. doi: 10.1016/j.phytochem.2005.11.017. Epub 2006 Jan 5.
Salicylic acid (SA) and jasmonic acid (JA) are plant hormones involved in basal resistance against plant pathogens and also in induced resistance. The aim of this study is to develop a fast and sensitive method to determine simultaneously the levels of both these hormones. The present paper proposes a method that includes hormone extraction with MeOH-H(2)O-HOAc (90:9:1, v/v), evaporation of the extracts, and injection into the liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) system in multiple reaction monitoring (MRM). Endogenous SA and JA levels in noninfested control cucumber cotyledons were 30.96 and 0.73ngg(-1) fresh weight, respectively. In roots, the levels were 8.31 and 15.82ngg(-1) FW, respectively. In plants treated with the biological control agent Trichoderma asperellum strain T-34, the levels of SA and JA did not differ from control plants. Rhizoctonia solani-diseased cucumber plants showed higher levels of SA and JA compared to noninfested controls (up to 2 and 13-fold higher, respectively). Detection limits for SA and JA were 0.45 and 0.47ngg(-1) fresh weight, respectively. The results of our research include the development of a method that is both fast and highly sensitive in the simultaneous quantitation of SA and JA from crude cucumber plant extracts, avoiding any purification and derivatization steps.
水杨酸（SA）和茉莉酸（JA）是参与植物对病原体的基础抗性以及诱导抗性的植物激素。本研究的目的是开发一种快速且灵敏的方法，以同时测定这两种激素的水平。本文提出了一种方法，该方法包括用甲醇 - 水 - 乙酸（90:9:1，v/v）提取激素，蒸发提取物，并以多反应监测（MRM）模式注入液相色谱 - 电喷雾电离串联质谱（LC - ESI - MS/MS）系统。未受侵染的对照黄瓜子叶中内源性SA和JA的水平分别为30.96和0.73 ng g⁻¹鲜重。在根中，水平分别为8.31和15.82 ng g⁻¹ FW。在用生防菌棘孢木霉菌株T - 34处理的植物中，SA和JA的水平与对照植物没有差异。与未受侵染的对照相比，立枯丝核菌病害黄瓜植株显示出更高水平的SA和JA（分别高达2倍和13倍）。SA和JA的检测限分别为0.45和0.47 ng g⁻¹鲜重。我们的研究结果包括开发了一种方法，该方法在从粗制黄瓜植物提取物中同时定量SA和JA时既快速又高度灵敏，避免了任何纯化和衍生化步骤。
