细胞壁降解酶在 对 的拮抗特性中的作用 。 你提供的原文似乎不完整，“against”后面缺少具体内容。
The Role of Cell Wall Degrading Enzymes in Antagonistic Traits of Against .
作者信息
Ghasemi Soleiman, Safaie Naser, Shahbazi Samira, Shams-Bakhsh Masoud, Askari Hamed
机构信息
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy organization of Iran (AEOI), Alborz, Iran.
出版信息
Iran J Biotechnol. 2020 Oct 1;18(4):e2333. doi: 10.30498/IJB.2020.2333. eCollection 2020 Oct.
BACKGROUND
High antagonistic ability of different species against a diverse range of plant pathogenic fungi has led them to be used as a biological fungicide in agriculture. They can also promote plant growth, fertility, resistance to stress, and absorption of nutrients. They are also opportunistic and symbiotic pathogens, which can lead to the activation of plant defense mechanisms.
OBJECTIVES
The aim of this present study was to investigate possible enhancement of lytic enzymes production and biocontrol activity of against through gamma radiation and to find the relationship between changes in lytic enzyme production and antagonistic activity of .
MATERIAL AND METHODS
Dual culture conditions were used to evaluate the antagonistic effect of and its gamma mutants against . Then, their chitinase and cellulase activities were measured. For more detailed investigation of enzymes, densitometry pattern of the proteins was extracted from the wild-type and its mutants were obtained via SDS-polyacrylamide gel electrophoresis.
RESULTS
The mutant T.vi M, wild-type and mutant T.vi M strains showed the maximum antagonistic effects against the pathogen, respectively. Data showed that the mutant T. vi M reduced the growth of by 58 %. The mutants revealed significantly different (p<0.05) protein contents, chitinase and cellulase production (mg.mL) and activity (U.mL-1) compared to the wild-type strain. The highest extracellular protein production in the supernatant of chitinase and cellulase TFM was observed for the T.vi M and T.vi M strains, respectively. The T.vi M and wild-type strains secreted chitinase and cellulase significantly more than other strains did. Densitometry of SDS-PAGE gel bands indicated that both the amount and diversity of chitinase related proteins in the selected mutant (T. vi M) were far higher than those of the wild-type. The diversity of molecular weight of proteins extracted from the M (20 proteins or bands) was very high compared to the wild-type (10 proteins) and mutant T.vi M (2 proteins).
CONCLUSIONS
Overall, there was a strong link between the diversity of various chitinase proteins and the antagonistic properties of the mutant M8.
背景
不同物种对多种植物病原真菌具有高度拮抗能力，这使得它们在农业中被用作生物杀菌剂。它们还能促进植物生长、提高肥力、增强抗逆性以及促进养分吸收。它们也是机会性和共生性病原菌，可导致植物防御机制的激活。
目的
本研究旨在通过γ射线辐射研究提高[具体菌种]对[目标病原菌]的裂解酶产量和生物防治活性的可能性，并找出裂解酶产量变化与[具体菌种]拮抗活性之间的关系。
材料与方法
采用双培养条件评估[具体菌种]及其γ突变体对[目标病原菌]的拮抗作用。然后，测定它们的几丁质酶和纤维素酶活性。为更详细地研究酶，通过SDS-聚丙烯酰胺凝胶电泳从[具体菌种]野生型及其突变体中提取蛋白质的光密度扫描图谱。
结果
突变体T.vi M、[具体菌种]野生型和突变体T.vi M菌株分别对病原菌表现出最大拮抗作用。数据显示，突变体T. vi M使[目标病原菌]的生长降低了58%。与野生型菌株相比，突变体的蛋白质含量、几丁质酶和纤维素酶产量（mg.mL）及活性（U.mL-1）有显著差异（p<0.05）。在几丁质酶和纤维素酶TFM的上清液中，分别观察到T.vi M和T.vi M菌株的胞外蛋白产量最高。T.vi M和野生型菌株分泌的几丁质酶和纤维素酶明显多于其他菌株。SDS-PAGE凝胶条带的光密度扫描表明，所选突变体（T. vi M）中几丁质酶相关蛋白的数量和多样性远高于野生型。从[具体菌种]M（20种蛋白质或条带）中提取的蛋白质分子量多样性与野生型（10种蛋白质）和突变体T.vi M（2种蛋白质）相比非常高。
结论
总体而言，各种几丁质酶蛋白的多样性与突变体M8的拮抗特性之间存在密切联系。
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