组合达普西染料库及其在人血清白蛋白位点选择性探针中的应用。
Combinatorial dapoxyl dye library and its application to site selective probe for human serum albumin.
作者信息
Min Jaeki, Lee Jae Wook, Ahn Young-Hoon, Chang Young-Tae
机构信息
Department of Chemistry, New York University, New York, New York 10003, USA.
出版信息
J Comb Chem. 2007 Nov-Dec;9(6):1079-83. doi: 10.1021/cc0700546. Epub 2007 Sep 29.
The combinatorial fluorescent dapoxyl dye library was prepared by both solution- and solid-phase synthesis, generating 80 unique dapoxyl derivatives. A fluorescence-based screening toward human serum albumin (HSA) found one highly sensitive HSA binder ( A41-S) with over 55-fold intensity change. Displacement assay showed the selective binding of A41-S to the site I of HSA, addressing its potential to be a highly selective and sensitive HSA probe.
通过溶液合成和固相合成制备了组合荧光达波西染料库，生成了80种独特的达波西衍生物。基于荧光对人血清白蛋白（HSA）进行筛选，发现一种高灵敏度的HSA结合剂（A41-S），其强度变化超过55倍。置换实验表明A41-S与HSA的位点I选择性结合，这表明它有潜力成为一种高选择性和高灵敏度的HSA探针。
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