Devamanoharan P S, Ramachandran S, Varma S D
Department of Ophthalmology, University of Maryland School of Medicine, Baltimore 21201.
Curr Eye Res. 1991 Sep;10(9):831-8. doi: 10.3109/02713689109013879.
Lenses from normal rabbits, mice, rats, cattle, guinea pigs, lambs, chicken, cats, baboons, blue acara (fish) and dogs were examined for the presence of H2O2. No previous reports exist on the presence and levels of H2O2 in normal eye lenses. Freshly isolated lenses of these animals were extracted with trichloroacetic acid and the extract neutralized with Tris. H2O2 was assayed in these extracts by reacting them with 1-14C-alpha-ketoglutarate and measuring the 14CO2 produced by peroxide-dependent decarboxylation. Peroxide of the order of 10(-4)M was detected in most of the lenses except in baboons wherein it exists between 10(-4) and 10(-5)M. Culture experiments with rat lenses demonstrated that GSH may make a major contribution to the formation of H2O2 in the intact lens in vivo.
检测了来自正常兔子、小鼠、大鼠、牛、豚鼠、羔羊、鸡、猫、狒狒、蓝鳃太阳鱼（鱼类）和狗的晶状体中过氧化氢的存在情况。此前尚无关于正常眼晶状体中过氧化氢的存在情况及含量水平的报道。这些动物刚分离出的晶状体用三氯乙酸提取，提取物用Tris中和。通过将这些提取物与1-14C-α-酮戊二酸反应，并测量过氧化物依赖性脱羧产生的14CO2，来检测其中的过氧化氢。除狒狒的晶状体中过氧化氢含量在10(-4)至10(-5)M之间外，大多数晶状体中检测到的过氧化物约为10(-4)M。对大鼠晶状体进行的培养实验表明，谷胱甘肽可能在体内完整晶状体中过氧化氢的形成中起主要作用。
