Devamanoharan P S, Varma S D
Department of Ophthalmology, University of Maryland at Baltimore 21201, USA.
Ophthalmic Res. 1995;27 Suppl 1:39-43. doi: 10.1159/000267837.
Hydrogen peroxide levels have been determined in rat lenses by using two methods, a chemiluminescent and a radioisotopic method. The average content was found to be 155 +/- 20 and 127 +/- 18 nmol/g wet weight of the tissue, respectively, by the two methods. The reaction of H2O2 with dichlorophenol-indophenol in the presence of peroxidase was also studied. However, this was found to be less suitable. The results of the chemiluminescent determinations are similar to the results with the radioisotopic methods demonstrating the feasibility of determining H2O2 by chemiluminescence measurement as well.
已通过两种方法测定了大鼠晶状体中的过氧化氢水平，一种是化学发光法，另一种是放射性同位素法。通过这两种方法分别测得组织湿重中过氧化氢的平均含量为155±20和127±18 nmol/g。还研究了在过氧化物酶存在的情况下，过氧化氢与二氯酚靛酚的反应。然而，发现这种方法不太适用。化学发光测定结果与放射性同位素法的结果相似，这也证明了通过化学发光测量来测定过氧化氢的可行性。
