[血管性痴呆大鼠海马中白细胞介素-1β和肿瘤坏死因子-α含量的变化及电针的干预作用]

[Changes of interleukin-1beta and TNF-alpha contents in the hippocampus and the interventional effect of electroacupuncture in vascular dementia rats].

作者信息

Li Wang, Lai Xin-Sheng

机构信息

Guangzhou University of Chinese Medicine, Guangzhou 510405, China.

出版信息

Zhen Ci Yan Jiu. 2007 Feb;32(1):34-7.

PMID:17580438

Abstract

OBJECTIVE

To observe the relationship between changes of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) contents in the hippocampus and learning-memory ability in vascular dementia (VD) rats and the interventional effect of electroacupuncture (EA) on the contents of IL-1beta and TNF-alpha.

METHODS

SD rats were randomized into control group (n = 10), model group (n = 11) and EA group (n = 12). VD model was established by using Pulsinelli's 4-vessel occlusion (4-VO) technique. EA (2 mA, 50 Hz) was applied to "Baihui" (GV 20) and "Dazhui" (GV 14) for 20 min, once a day and continuously for 15 days. The rats' learning-memory abilities were tested by Morris water-maze (place navigation test and special probe test) continuously for 6 days, and then the rats were sacrificed for taking hippocampus tissue which was homogenized for detecting IL-1beta and TNF-alpha contents by using radioimmunoassay (RIA) technique.

RESULTS

Place navigation test and special probe test showed that compared with control group, the mean escape latencies and the times of crossing the original platform quadrant in model group and EA group were significantly longer and more (P < 0.01). Comparison between model group and EA group indicated that the escape latency of the later group was markedly shorter than that of the former group (P < 0.05), no significant differences were found between EA group and control group in the staying duration in the original platform quadrant (P > 0.05), suggesting an improvement of learning-memory abilities after EA. In comparison with control group, both IL-1beta and TNF-alpha contents in the hippocampus tissue were significantly higher in model group and EA group (P < 0.05, 0.01), and those of EA group were considerably lower than those of model group (P < 0.01).

CONCLUSION

EA can improve VD rats' learning-memory abilities, which is closely associated with its effects in suppressing VD-induced increase of IL-1beta and TNF-alpha levels in the hippocampus.

摘要

目的

观察血管性痴呆（VD）大鼠海马组织中白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）含量变化与学习记忆能力的关系，以及电针（EA）对IL-1β和TNF-α含量的干预作用。

方法

将SD大鼠随机分为对照组（n = 10）、模型组（n = 11）和电针组（n = 12）。采用Pulsinelli四血管闭塞（4-VO）技术建立VD模型。将电针（2 mA，50 Hz）施加于“百会”（GV 20）和“大椎”（GV 14）20分钟，每天1次，连续15天。连续6天通过Morris水迷宫（位置导航试验和特殊探针试验）测试大鼠的学习记忆能力，然后处死大鼠取海马组织，匀浆后采用放射免疫分析（RIA）技术检测IL-1β和TNF-α含量。

结果

位置导航试验和特殊探针试验显示，与对照组相比，模型组和电针组的平均逃避潜伏期及穿越原平台象限的次数显著延长和增多（P < 0.01）。模型组与电针组比较，电针组的逃避潜伏期明显短于模型组（P < 0.05），电针组与对照组在原平台象限的停留时间无显著差异（P > 0.05），提示电针后学习记忆能力有所改善。与对照组相比，模型组和电针组海马组织中IL-1β和TNF-α含量均显著升高（P < 0.05，0.01），且电针组的含量明显低于模型组（P < 0.01）。