属于Pfam DUF178的两种蛋白质的X射线结构显示出与Pfam DUF191家族意想不到的结构相似性。
X-ray structures of two proteins belonging to Pfam DUF178 revealed unexpected structural similarity to the DUF191 Pfam family.
作者信息
Tyagi Rajiv, Burley Stephen K, Swaminathan Subramanyam
机构信息
Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
出版信息
BMC Struct Biol. 2007 Oct 1;7:62. doi: 10.1186/1472-6807-7-62.
BACKGROUND
Pfam is a comprehensive collection of protein domains and families, with a range of well-established information including genome annotation. Pfam has two large series of functionally uncharacterized families, known as Domains of Unknown Function (DUFs) and Uncharacterized Protein Families (UPFs).
RESULTS
Crystal structures of two proteins from Deinococcus radiodurans and Streptomyces coelicolor belonging to Pfam protein family DUF178 (ID: PF02621) have been determined using Selenium-Single-wavelength Anomalous Dispersion (Se-SAD). Based on the structure, we have identified the putative function for this family of protein.
CONCLUSION
Unexpectedly, we found that DUF178 Pfam is remarkably similar to Pfam family DUF191 suggesting that the sequence-based classification alone may not be sufficient to classify proteins into Pfam families.
背景
Pfam是蛋白质结构域和家族的综合集合，拥有一系列完善的信息，包括基因组注释。Pfam有两个功能未明确的大家族，即未知功能结构域（DUF）和未表征蛋白质家族（UPF）。
结果
利用硒单波长反常散射（Se-SAD）确定了来自耐辐射球菌和天蓝色链霉菌的属于Pfam蛋白质家族DUF178（ID：PF02621）的两种蛋白质的晶体结构。基于该结构，我们确定了该蛋白质家族的推定功能。
结论
出乎意料的是，我们发现Pfam家族DUF178与Pfam家族DUF191非常相似，这表明仅基于序列的分类可能不足以将蛋白质分类到Pfam家族中。
Zotero 插件