Lee Hae-Won, Yu Hong-Shan, Liu Qing-Mei, Jung Hae-Min, An Dong-Shan, Im Wan-Taek, Jin Feng-Xie, Lee Sung-Taik
Environmental and Molecular Microbiology Laboratory, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, South Korea.
College of Bio & Food Technology, Dalian Polytechnic University, Qinggong-yuan No. 1, Ganjingzi-qu, Dalian 116034, P. R. China.
Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2280-2283. doi: 10.1099/ijs.0.65023-0.
A Gram-negative, chemo-organotrophic, non-motile, non-spore-forming, rod-shaped bacterium (designated strain Ko04(T)) was isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor, and was investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Ko04(T) belongs to the order Rhizobiales in the Alphaproteobacteria. Comparative 16S rRNA gene sequence analysis showed that strain Ko04(T) was most closely related to Kaistia adipata (97.5 %) and that sequence similarities with other species of Rhizobiales with validly published names were less than 92.5 %. The predominant ubiquinone was Q-10 and the major fatty acids were C(18 : 1)omega7c/omega9t/omega12t, C(19 : 0 )cyclo omega8c and C(18 : 0). The G+C content of the genomic DNA of strain Ko04(T) was 67.8 mol%. The level of DNA-DNA relatedness with K. adipata Chj404(T) was 15 %. The results of the genotypic analyses in combination with chemotaxonomic and physiological data demonstrated that strain Ko04(T) represents a novel species within the genus Kaistia, for which the name Kaistia granuli sp. nov. is proposed. The type strain is Ko04(T) (=KCTC 12575(T)=LMG 23410(T)).
从升流式厌氧污泥床反应器的厌氧颗粒中分离出一株革兰氏阴性、化能有机营养型、无运动性、不形成芽孢的杆状细菌（命名为菌株Ko04(T)），并采用多相分类方法对其进行了研究。基于16S rRNA基因序列的系统发育分析表明，菌株Ko04(T)属于α-变形菌纲的根瘤菌目。比较16S rRNA基因序列分析表明，菌株Ko04(T)与脂肪凯斯氏菌（Kaistia adipata）关系最为密切（相似度为97.5%），与其他已有效发表名称的根瘤菌目物种的序列相似度低于92.5%。主要泛醌为Q-10，主要脂肪酸为C(18 : 1)ω7c/ω9t/ω12t、C(19 : 0)环ω8c和C(18 : 0)。菌株Ko04(T)基因组DNA的G+C含量为67.8 mol%。与脂肪凯斯氏菌Chj404(T)的DNA-DNA杂交率为15%。基因型分析结果与化学分类和生理数据相结合表明，菌株Ko04(T)代表凯斯氏菌属内的一个新物种，为此提出新名称凯斯氏菌颗粒种（Kaistia granuli sp. nov.）。模式菌株为Ko04(T)（=KCTC 12575(T)=LMG 23410(T)）。
