Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong-gu, Daejeon 305-333, Republic of Korea.
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):201-204. doi: 10.1099/ijs.0.018689-0. Epub 2010 Feb 26.
A Gram-reaction-negative, chemo-organotrophic, non-motile, non-spore-forming, rod-shaped bacterium (strain Gsoil 080(T)) was isolated from soil collected in a ginseng field in Pocheon Province, South Korea, and was investigated by using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 080(T) was related most closely to Inquilinus limosus strains AU0476(T) and AU1979 (98.9 % similarity to both). Strain Gsoil 080(T) shared ≤91.3 % 16S rRNA gene sequence similarity with the type strains of other recognized species examined. The genus Inquilinus belongs to the family Rhodospirillaceae in the order Rhodospirillales, class Alphaproteobacteria. The predominant ubiquinone was Q-10 and the major fatty acids were summed feature 7 (C(18 : 1)ω9c/ω12t/ω7c) and C(19 : 0) cyclo ω8c. The G+C content of the genomic DNA of strain Gsoil 080(T) was 69.9 mol%. The level of DNA-DNA relatedness between strain Gsoil 080(T) and I. limosus LMG 20952(T) was 12 %. The results of genotypic analyses in combination with chemotaxonomic and physiological data demonstrated that strain Gsoil 080(T) represents a novel species of the genus Inquilinus, for which the name Inquilinus ginsengisoli sp. nov. is proposed. The type strain is Gsoil 080(T) (=KCTC 12574(T) =LMG 23638(T)).
一株革兰氏反应阴性、化能有机营养型、非运动、非孢子形成、杆状细菌（Gsoil 080(T) 株）从韩国抱川人参种植地的土壤中分离得到，并采用多相分类学方法进行了研究。比较 16S rRNA 基因序列分析表明，Gsoil 080(T) 株与 Inquilinus limosus 菌株 AU0476(T) 和 AU1979 最为密切相关（与两者的相似度均为 98.9%）。Gsoil 080(T) 株与其他经鉴定的种的模式菌株的 16S rRNA 基因序列相似性均≤91.3%。Inquilinus 属属于红螺旋菌科的红螺旋菌目，α变形菌纲。该属的主要醌类为 Q-10，主要脂肪酸为 C(18 : 1)ω9c/ω12t/ω7c 和 C(19 : 0) 环 ω8c。Gsoil 080(T) 株的基因组 DNA G+C 含量为 69.9 mol%。Gsoil 080(T) 株与 I. limosus LMG 20952(T) 之间的 DNA-DNA 同源性为 12%。基因型分析结果结合化学分类和生理数据表明，Gsoil 080(T) 株代表了 Inquilinus 属的一个新种，建议将其命名为人参土壤栖居的 Inquilinus ginsengisoli sp. nov.。其模式菌株为 Gsoil 080(T)（=KCTC 12574(T)=LMG 23638(T)）。
