Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
Agricultural Microbiology, Department of Agricultural Microbiology, Agricultural College and Research Institute, Madurai India.
Int J Syst Evol Microbiol. 2010 Apr;60(Pt 4):949-952. doi: 10.1099/ijs.0.011924-0. Epub 2009 Aug 6.
An ivory-coloured bacterium, designated strain 5YN7-3(T), was isolated from a wetland, Yongneup, Korea. Cells of the strain were aerobic, Gram-stain-negative, non-motile and short rods. 16S rRNA gene sequence analysis demonstrated that strain 5YN7-3(T) belongs to the order Rhizobiales of the class Alphaproteobacteria and is closely related to Kaistia soli 5YN9-8(T) (97.8 %), Kaistia granuli Ko04(T) (97.6 %) and Kaistia adipata Chj404(T) (97.4 %). Strain 5YN7-3(T) showed DNA-DNA hybridization values of 28, 22 and 35 % with K. granuli Ko04(T), K. soli 5YN9-8(T) and K. adipata Chj404(T), respectively. The major fatty acids were C(18 : 1)omega7c (51.2 %), C(19 : 0) cyclo omega8c (25.0 %), C(18 : 0) (12.9 %) and C(16 : 0) (10.8 %) (>10 % of total fatty acids). Ubiquinone-10 was the major isoprenoid quinone and the DNA G+C content was 66.5 mol%. The phenotypic characteristics in combination with 16S rRNA gene sequence analysis and DNA-DNA hybridization data clearly define strain 5YN7-3(T) as a novel species of the genus Kaistia, for which the name Kaistia terrae sp. nov. is proposed. The type strain is 5YN7-3(T) (=KACC 12910(T) =DSM 21341(T)).
一株象牙色的细菌，被命名为 5YN7-3(T) 株，从韩国永泥浦湿地中分离得到。该菌株细胞为需氧、革兰氏阴性、非运动和短杆状。16S rRNA 基因序列分析表明，菌株 5YN7-3(T) 属于α变形菌纲根瘤菌目，与 Kaistia soli 5YN9-8(T)（97.8%）、Kaistia granuli Ko04(T)（97.6%）和 Kaistia adipata Chj404(T)（97.4%）最为密切相关。菌株 5YN7-3(T) 与 Kaistia granuli Ko04(T)、Kaistia soli 5YN9-8(T) 和 Kaistia adipata Chj404(T) 的 DNA-DNA 杂交值分别为 28%、22%和 35%。主要脂肪酸为 C(18:1)omega7c（51.2%）、C(19:0)cyclo omega8c（25.0%）、C(18:0)（12.9%）和 C(16:0)（10.8%）（>10%的总脂肪酸）。泛醌-10 为主要异戊二烯醌，DNA G+C 含量为 66.5mol%。表型特征结合 16S rRNA 基因序列分析和 DNA-DNA 杂交数据，明确将菌株 5YN7-3(T) 定义为 Kaistia 属的一个新种，命名为 Kaistia terrae sp. nov.。模式菌株为 5YN7-3(T)（=KACC 12910(T)=DSM 21341(T)）。
