维生素D受体基因多态性与慢性肾脏病及牙周炎易感性之间的关联。

Association between vitamin D receptor gene polymorphisms and susceptibility to chronic kidney disease and periodontitis.

作者信息

de Souza Cleber Machado, Braosi Ana Paula Ribeiro, Luczyszyn Sônia Mara, Avila Andréa Rodrigues, de Brito Rui Barbosa, Ignácio Sérgio Aparecido, Probst Christian Macagnan, Riella Miguel Carlos, Sotomaior Vanessa Santos, Mira Marcelo Távora, Pecoits-Filho Roberto, Trevilatto Paula Cristina

机构信息

Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.

出版信息

Blood Purif. 2007;25(5-6):411-9. doi: 10.1159/000109235. Epub 2007 Oct 3.

DOI:10.1159/000109235

PMID:17914260

Abstract

BACKGROUND/AIMS: Chronic kidney disease (CKD) and periodontitis (PD) are serious public-health concerns. Vitamin D is a fat-soluble steroid hormone that interacts with its nuclear receptor (VDR) to regulate a variety of biological processes, such as bone metabolism, immune response modulation and transcription of several genes involved in CKD and PD disease mechanisms. The aim of this work was to investigate the association between polymorphisms in the VDR gene and end-stage renal disease (ESRD) and PD.

METHODS

222 subjects with and without ESRD (in hemodialysis) were divided into groups with and without PD. Polymorphisms TaqI and BsmI in the VDR gene were analyzed by PCR restriction fragment length polymorphism. The significance of differences in allele, genotype and haplotype frequencies between groups was assessed by the chi2 test (p value <0.05) and odds ratio (OR).

RESULTS

Allele G was associated with protection against ESRD: groups without versus with ESRD (GG) x (GA+AA): OR = 2.5, 95% CI = 1.4-4.6, p = 0.00; (G x A): OR = 1.5, 95% CI = 1.0-2.3, p = 0.02; (TG + CG) x (TA + CA): OR = 1.5, 95% CI = 1.0-2.3, p = 0.02. No association was observed between the study polymorphisms and susceptibility to or protection against PD.

CONCLUSION

Allele G of the VDR BsmI polymorphism was associated with protection against ESRD.

摘要

背景/目的：慢性肾脏病（CKD）和牙周炎（PD）是严重的公共卫生问题。维生素D是一种脂溶性类固醇激素，它与其核受体（VDR）相互作用，调节多种生物学过程，如骨代谢、免疫反应调节以及参与CKD和PD疾病机制的多个基因的转录。本研究旨在探讨VDR基因多态性与终末期肾病（ESRD）和PD之间的关联。

方法

222例有或无ESRD（接受血液透析）的受试者被分为有或无PD的组。通过聚合酶链反应-限制性片段长度多态性分析VDR基因中的TaqI和BsmI多态性。采用卡方检验（p值<0.05）和比值比（OR）评估组间等位基因、基因型和单倍型频率差异的显著性。

结果

等位基因G与预防ESRD相关：无ESRD组与有ESRD组相比（GG）×（GA + AA）：OR = 2.5，95%可信区间（CI）= 1.4 - 4.6，p = 0.00；（G×A）：OR = 1.5，95%CI = 1.0 - 2.3，p = 0.02；（TG + CG）×（TA + CA）：OR = 1.5，95%CI = 1.0 - 2.3，p = 0.02。未观察到所研究的多态性与PD易感性或预防之间存在关联。