El Jilani Mouna M, Mohamed Abdenaser A, Zeglam Hamza Ben, Alhudiri Inas M, Ramadan Ahmad M, Saleh Saleh S, Elkabir Mohamed, Amer Ibrahim Ben, Ashammakhi Nureddin, Enattah Nabil S
Department of Biomedical Engineering, Division of Genetic Engineering, School of Engineering and Applied Science, Libyan Academy, Tripoli, Libya.
Department of Genetic Engineering, Biotechnology Research Center (BTRC), Twisha, Tripoli, Libya.
Libyan J Med. 2015 Mar 19;10(1):26771. doi: 10.3402/ljm.v10.26771. eCollection 2015.
Chronic periodontitis (CP) is a common oral disease characterized by inflammation in the supporting tissue of the teeth 'the periodontium', periodontal attachment loss, and alveolar bone loss. The disease has a microbial etiology; however, recent findings suggest that the genetic factors, such as vitamin D receptor (VDR) gene polymorphisms, have also been included.
Investigation of the relationship between VDR gene polymorphisms and CP among Libyans.
In this study, we examined 196 unrelated Libyans between the ages of 25 and 65 years, including 99 patients and 97 controls. An oral examination based on Ramfjord Index was performed at different dental clinics in Tripoli and information were collected using a self-reported questionnaire. DNA was extracted from buccal swabs; the VDR ApaI, BsmI, and FokI polymorphisms were genotyped using polymerase chain reaction and were sequenced using Sanger Method.
A significant difference in the newly detected ApaI SNP C/T rs#731236 was found (p=0.022), whereas no significant differences were found in ApaI SNP G/T rs#7975232, BsmI SNP A/G rs#1544410, and FokI SNP A/G rs#2228570 between patients and controls (p=0.939, 0.466, 0.239), respectively.
VDR ApaI SNP C/T rs#731236 may be related to the risk of CP in the Libyan population.
慢性牙周炎（CP）是一种常见的口腔疾病，其特征为牙齿支持组织（牙周组织）的炎症、牙周附着丧失和牙槽骨丧失。该疾病具有微生物病因；然而，最近的研究结果表明，遗传因素，如维生素D受体（VDR）基因多态性，也与之相关。
调查利比亚人中VDR基因多态性与慢性牙周炎之间的关系。
在本研究中，我们检查了196名年龄在25至65岁之间的无亲属关系的利比亚人，其中包括99名患者和97名对照。在的黎波里的不同牙科诊所基于Ramfjord指数进行了口腔检查，并使用自填问卷收集信息。从口腔拭子中提取DNA；使用聚合酶链反应对VDR ApaI、BsmI和FokI多态性进行基因分型，并使用桑格法进行测序。
新检测到的ApaI SNP C/T rs#731236存在显著差异（p = 0.022），而患者与对照之间在ApaI SNP G/T rs#7975232、BsmI SNP A/G rs#1544410和FokI SNP A/G rs#2228570方面未发现显著差异（p分别为0.939、0.466、0.239）。
VDR ApaI SNP C/T rs#731236可能与利比亚人群患慢性牙周炎的风险有关。
