多聚血红蛋白-纤维蛋白原：一种在血液稀释环境中具有类似血小板特性的新型氧载体。
Polyhemoglobin-fibrinogen: a novel oxygen carrier with platelet-like properties in a hemodiluted setting.
作者信息
Wong Naomi S W, Chang Thomas M S
机构信息
Artificial Cells and Organs Research Centre, Department of Physiology, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
出版信息
Artif Cells Blood Substit Immobil Biotechnol. 2007;35(5):481-9. doi: 10.1080/10731190701586210.
Polyhemoglobin (polyHb) is currently being assessed in phase III trials under various formulations. At present, none contain clotting factors or platelet substitutes to aid in hemostasis. We have prepared a novel blood substitute that is an oxygen carrier with platelet-like activity. This is formed by crosslinking fibrinogen to hemoglobin to form polyhemoglobin-fibrinogen (polyHb-Fg). This was studied and compared to polyHb for its effect on coagulation both in vitro and in vivo. In the in vitro experiments, PolyHb-Fg showed similar clotting times as whole blood, whereas polyHb showed significantly higher clotting times. This result was confirmed in in vivo experiments using an exchange transfusion rat-model. Using PolyHb, exchange transfusion of 80% or more increased the normal clotting time (1-2 mins) to > 10 mins. Partial clots formed with PolyHb did not adhere to the tubing wall. With PolyHb-Fg, a normal clotting time is maintained, even with 98% exchange transfusion.
多聚血红蛋白（polyHb）目前正在多种制剂下进行III期试验评估。目前，没有一种制剂含有凝血因子或血小板替代物来辅助止血。我们制备了一种新型血液替代品，它是一种具有血小板样活性的氧载体。这是通过将纤维蛋白原与血红蛋白交联形成多聚血红蛋白-纤维蛋白原（polyHb-Fg）而形成的。研究了其对体外和体内凝血的影响，并与多聚血红蛋白进行了比较。在体外实验中，polyHb-Fg的凝血时间与全血相似，而多聚血红蛋白的凝血时间显著更长。使用换血大鼠模型的体内实验证实了这一结果。使用多聚血红蛋白，80%或更多的换血会使正常凝血时间（1-2分钟）增加到>10分钟。用多聚血红蛋白形成的部分凝块不粘附在管壁上。使用polyHb-Fg，即使进行98%的换血，也能维持正常的凝血时间。
Zotero 插件