Cui Rongjing, Pan Hong-Cheng, Zhu Jun-Jie, Chen Hong-Yuan
Key Lab of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
Anal Chem. 2007 Nov 15;79(22):8494-501. doi: 10.1021/ac070923d. Epub 2007 Oct 10.
A versatile immunosensor using CdTe quantum dots as electrochemical and fluorescent labels has been developed for sensitive protein detection. This sandwich-type sensor is fabricated on an indium tin oxide chip covered with a well-ordered gold nanoparticle monolayer. Gel imaging systems were successfully introduced to develop a novel high-efficient optical immunoassay, which could perform simultaneous detection for the samples with a series of different concentrations of a target analyte. The linear range of this assay was between 0.1 and 500 ng/mL, and the assay sensitivity could be further increased to 0.005 ng/mL with the linear range from 0.005 to 100 ng/mL by stripping voltammetric analysis. The immunosensor showed good precision, high sensitivity, acceptable stability, and reproducibility and could be used for the detection of real sample with consistent results in comparison with those obtained by the ELISA method.
一种以碲化镉量子点作为电化学和荧光标记物的多功能免疫传感器已被开发用于灵敏的蛋白质检测。这种三明治型传感器制备在覆盖有有序金纳米颗粒单层的氧化铟锡芯片上。成功引入凝胶成像系统以开发一种新型高效光学免疫分析方法，该方法可对含有一系列不同浓度目标分析物的样品进行同时检测。该分析方法的线性范围为0.1至500 ng/mL，通过溶出伏安分析，分析灵敏度可进一步提高至0.005 ng/mL，线性范围为0.005至100 ng/mL。该免疫传感器具有良好的精密度、高灵敏度、可接受的稳定性和重现性，并且与酶联免疫吸附测定法（ELISA）相比，可用于实际样品检测且结果一致。
