阿霉素诱导的大鼠伤口愈合损伤恢复的时间变化。
Temporal variation in the recovery from impairment in adriamycin-induced wound healing in rats.
作者信息
Alagol Haluk, Dinc Soykan, Basgut Bilgen, Abacioglu Nurettin
机构信息
Department of Pharmacology, Gazi University, 06330, Hipodrom, Ankara, Turkey.
出版信息
J Circadian Rhythms. 2007 Oct 10;5:6. doi: 10.1186/1740-3391-5-6.
BACKGROUND
An adriamycin-induced impairment of wound healing has been demonstrated experimentally in rats. The purpose of this study is to investigate a possible temporal variation in recovery from the impairment of wound healing caused by adriamycin administration.
METHODS
The subjects were 120 female Spraque-Dawley rats. They were divided into eight groups, undergoing adriamycin administration (8 mg/kg, i.v.) at 9 a.m. or 9 p.m. on day 0 and laparotomy on day 0, 7, 14 or 21. Blast pressures were recorded after the incision line had been opened, and tissue samples were kept at -30 degrees C for later measurement of hydroxyproline levels.
RESULTS
Adriamycin treatment in rats at 9 p.m. resulted in significantly lower blast pressure levels than treatment at 9 a.m. between days 7 and 21, indicating a lag effect of healing time in wounded tissues. However the decreased hydroxyproline levels were not changed at these days and sessions.
CONCLUSION
It is concluded that adriamycin-induced impairment of wound healing in adult female rats exhibits nycthemeral variation.
背景
实验已证实阿霉素可导致大鼠伤口愈合受损。本研究旨在探讨阿霉素所致伤口愈合受损恢复过程中可能存在的时间变化。
方法
选取120只雌性Spraque-Dawley大鼠，分为8组，于第0天上午9点或晚上9点静脉注射阿霉素（8mg/kg），并在第0、7、14或21天进行剖腹手术。切开切口线后记录爆破压力，组织样本保存在-30℃以备后续检测羟脯氨酸水平。
结果
晚上9点给予大鼠阿霉素治疗，在第7至21天期间，其爆破压力水平显著低于上午9点治疗组，表明受伤组织愈合时间存在滞后效应。然而，在这些天数和时间段内，羟脯氨酸水平的降低并未改变。
结论
得出结论，阿霉素诱导的成年雌性大鼠伤口愈合受损呈现昼夜变化。
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