Tu Min, Lu Bao-Rong, Zhu Youyong, Wang Yunyue
Ministry of Education Key Laboratory of Agricultural Biodiversity for Plant Disease Management, Key Laboratory of Plant Pathology, Yunnan Agricultural University, Kunming, P.R. China.
Biochem Genet. 2007 Dec;45(11-12):789-801. doi: 10.1007/s10528-007-9118-6. Epub 2007 Oct 11.
In order to estimate genetic diversity of rice (Oryza sativa L.) germplasm in Yunnan Province of China, 60 varieties from different regions were analyzed by microsatellite (SSR) fingerprints. Nine selected SSR primer pairs amplified a total of 55 alleles from these varieties, and high genetic diversity (0.706) was found, although it was not evenly distributed across the regions. Marked genetic variation was detected within the traditional varieties. A UPGMA dendrogram based on SSR polymorphism indicated a great variation among the rice varieties, with coefficients ranging between 0.229 and 1.000. The formation of the rice diversity pattern in Yunnan is associated with natural conditions and especially with diverse cultural demands and farming styles. Strategic conservation of rice germplasm in Yunnan is important, and this could be implemented by collecting varieties across geographic regions with sufficient individuals within the same varieties. Effective rice conservation should also consider cultural aspects during collection.
为了评估中国云南省水稻（Oryza sativa L.）种质资源的遗传多样性，利用微卫星（SSR）指纹图谱技术对来自不同地区的60个品种进行了分析。9对选定的SSR引物对这些品种共扩增出55个等位基因，结果发现遗传多样性较高（0.706），尽管其在各地区的分布并不均匀。在传统品种中检测到显著的遗传变异。基于SSR多态性构建的UPGMA聚类图表明水稻品种间存在很大差异，系数范围在0.229至1.000之间。云南水稻多样性模式的形成与自然条件有关，特别是与多样的文化需求和耕作方式有关。对云南水稻种质资源进行战略性保护很重要，这可以通过在不同地理区域收集品种并确保同一品种内有足够数量的个体来实现。有效的水稻保护在收集过程中还应考虑文化因素。
